α-lipoic acid reduces congenital malformations in the offspring of diabetic mice

Yoshihisa Sugimura, T. Murase, K. Kobayashi, K. Oyama, S. Hayasaka, Y. Kanou, Y. Oiso, Y. Murata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: The mechanism of diabetes-induced congenital malformation remains to be elucidated. It has been reported that α-lipoic acid (LA) prevents neural tube defects (NTDs) in offsprings of rats with streptozotocin-induced diabetes. Here, we evaluate the protective effect of LA against diabetic embryopathy, including NTDs, cardiovascular malformations (CVMs), and skeletal malformations, in mice. Methods: Female mice were rendered hyperglycemic using streptozotocin and then mated with normal male mouse. Pregnant diabetic or non-diabetic mice were treated daily with either LA (100 mg/kg body weight) or saline between gestational days 0 and 18. On day 18, fetuses were examined for congenital malformations. Results: Plasma glucose levels on day 18 were not affected by LA treatment. No congenital malformations were observed either in the saline-treated or LA-treated non-diabetic group. In the saline-treated diabetic group, 39% of fetuses had external malformations and 30% had NTDs. In the LA-treated diabetic group, the corresponding proportions were 11 and 8%, respectively. LA treatment also decreased the incidence of CVMs from 30-3% and of skeletal malformations from 29-6%. Conclusions: We conclude that LA can reduce NTDs, CVMs and skeletal malformations in the offspring of diabetic mice at term delivery.

Original languageEnglish
Pages (from-to)287-294
Number of pages8
JournalDiabetes/Metabolism Research and Reviews
Volume25
Issue number3
DOIs
Publication statusPublished - 04-06-2009

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Thioctic Acid
Neural Tube Defects
Fetus
Fetal Diseases
Experimental Diabetes Mellitus
Streptozocin
Body Weight
Glucose
Incidence

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Sugimura, Yoshihisa ; Murase, T. ; Kobayashi, K. ; Oyama, K. ; Hayasaka, S. ; Kanou, Y. ; Oiso, Y. ; Murata, Y. / α-lipoic acid reduces congenital malformations in the offspring of diabetic mice. In: Diabetes/Metabolism Research and Reviews. 2009 ; Vol. 25, No. 3. pp. 287-294.
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abstract = "Background: The mechanism of diabetes-induced congenital malformation remains to be elucidated. It has been reported that α-lipoic acid (LA) prevents neural tube defects (NTDs) in offsprings of rats with streptozotocin-induced diabetes. Here, we evaluate the protective effect of LA against diabetic embryopathy, including NTDs, cardiovascular malformations (CVMs), and skeletal malformations, in mice. Methods: Female mice were rendered hyperglycemic using streptozotocin and then mated with normal male mouse. Pregnant diabetic or non-diabetic mice were treated daily with either LA (100 mg/kg body weight) or saline between gestational days 0 and 18. On day 18, fetuses were examined for congenital malformations. Results: Plasma glucose levels on day 18 were not affected by LA treatment. No congenital malformations were observed either in the saline-treated or LA-treated non-diabetic group. In the saline-treated diabetic group, 39{\%} of fetuses had external malformations and 30{\%} had NTDs. In the LA-treated diabetic group, the corresponding proportions were 11 and 8{\%}, respectively. LA treatment also decreased the incidence of CVMs from 30-3{\%} and of skeletal malformations from 29-6{\%}. Conclusions: We conclude that LA can reduce NTDs, CVMs and skeletal malformations in the offspring of diabetic mice at term delivery.",
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Sugimura, Y, Murase, T, Kobayashi, K, Oyama, K, Hayasaka, S, Kanou, Y, Oiso, Y & Murata, Y 2009, 'α-lipoic acid reduces congenital malformations in the offspring of diabetic mice', Diabetes/Metabolism Research and Reviews, vol. 25, no. 3, pp. 287-294. https://doi.org/10.1002/dmrr.947

α-lipoic acid reduces congenital malformations in the offspring of diabetic mice. / Sugimura, Yoshihisa; Murase, T.; Kobayashi, K.; Oyama, K.; Hayasaka, S.; Kanou, Y.; Oiso, Y.; Murata, Y.

In: Diabetes/Metabolism Research and Reviews, Vol. 25, No. 3, 04.06.2009, p. 287-294.

Research output: Contribution to journalArticle

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T1 - α-lipoic acid reduces congenital malformations in the offspring of diabetic mice

AU - Sugimura, Yoshihisa

AU - Murase, T.

AU - Kobayashi, K.

AU - Oyama, K.

AU - Hayasaka, S.

AU - Kanou, Y.

AU - Oiso, Y.

AU - Murata, Y.

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N2 - Background: The mechanism of diabetes-induced congenital malformation remains to be elucidated. It has been reported that α-lipoic acid (LA) prevents neural tube defects (NTDs) in offsprings of rats with streptozotocin-induced diabetes. Here, we evaluate the protective effect of LA against diabetic embryopathy, including NTDs, cardiovascular malformations (CVMs), and skeletal malformations, in mice. Methods: Female mice were rendered hyperglycemic using streptozotocin and then mated with normal male mouse. Pregnant diabetic or non-diabetic mice were treated daily with either LA (100 mg/kg body weight) or saline between gestational days 0 and 18. On day 18, fetuses were examined for congenital malformations. Results: Plasma glucose levels on day 18 were not affected by LA treatment. No congenital malformations were observed either in the saline-treated or LA-treated non-diabetic group. In the saline-treated diabetic group, 39% of fetuses had external malformations and 30% had NTDs. In the LA-treated diabetic group, the corresponding proportions were 11 and 8%, respectively. LA treatment also decreased the incidence of CVMs from 30-3% and of skeletal malformations from 29-6%. Conclusions: We conclude that LA can reduce NTDs, CVMs and skeletal malformations in the offspring of diabetic mice at term delivery.

AB - Background: The mechanism of diabetes-induced congenital malformation remains to be elucidated. It has been reported that α-lipoic acid (LA) prevents neural tube defects (NTDs) in offsprings of rats with streptozotocin-induced diabetes. Here, we evaluate the protective effect of LA against diabetic embryopathy, including NTDs, cardiovascular malformations (CVMs), and skeletal malformations, in mice. Methods: Female mice were rendered hyperglycemic using streptozotocin and then mated with normal male mouse. Pregnant diabetic or non-diabetic mice were treated daily with either LA (100 mg/kg body weight) or saline between gestational days 0 and 18. On day 18, fetuses were examined for congenital malformations. Results: Plasma glucose levels on day 18 were not affected by LA treatment. No congenital malformations were observed either in the saline-treated or LA-treated non-diabetic group. In the saline-treated diabetic group, 39% of fetuses had external malformations and 30% had NTDs. In the LA-treated diabetic group, the corresponding proportions were 11 and 8%, respectively. LA treatment also decreased the incidence of CVMs from 30-3% and of skeletal malformations from 29-6%. Conclusions: We conclude that LA can reduce NTDs, CVMs and skeletal malformations in the offspring of diabetic mice at term delivery.

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