TY - JOUR
T1 - β-amyloid (25-35) enhances lipid metabolism and protein ubiquitination in cultured neurons
AU - Cazzaniga, Emanuela
AU - Bulbarelli, Alessandra
AU - Cassetti, Arianna
AU - Lonati, Elena
AU - Re, Francesca
AU - Palestini, Paola
AU - Mutoh, Tatsuro
AU - Masserini, Massimo
PY - 2007/8/1
Y1 - 2007/8/1
N2 - We investigated the effect of β-amyloid (Aβ) (25-35), a cytotoxic fragment of Aβ peptide, on lipid metabolism and protein ubiquitination in cultured rat hippocampal neurons. After treatment with Aβ under conditions leading to apoptotis, as assessed by caspase activity assay, the total cell mass of lipids changed following a biphasic behavior, with an increase that reached a maximum after 16 hr of treatment, followed by a decrease. The increase at 16 hr was 15.3% in the case of phospholipids and 103.0% in the case of gangliosides and was due to enhanced biosynthesis as confirmed by increase of radioactivity incorporation (phospholipids +52.0%, gangliosides +193.1%) in cells fed with tritiated palmitic acid. No change with respect to cholesterol was observed. Strikingly, under these conditions, the ubiquitination state of cell proteins strongly increased. These effects were not observed with the (35-25) reverse sequence peptide. Similarly to Aβ, lactacystin treatment increased lipid synthesis and protein ubiquitination; only lactacystin, and not Aβ, induced a strong decrease of proteasome chimotrypsin activity. These results suggest that Aβ enhances protein ubiquitination, without inhibiting proteasomal activity, and lipid synthesis. These results may shed new light on the mechanisms of Aβ toxicity.
AB - We investigated the effect of β-amyloid (Aβ) (25-35), a cytotoxic fragment of Aβ peptide, on lipid metabolism and protein ubiquitination in cultured rat hippocampal neurons. After treatment with Aβ under conditions leading to apoptotis, as assessed by caspase activity assay, the total cell mass of lipids changed following a biphasic behavior, with an increase that reached a maximum after 16 hr of treatment, followed by a decrease. The increase at 16 hr was 15.3% in the case of phospholipids and 103.0% in the case of gangliosides and was due to enhanced biosynthesis as confirmed by increase of radioactivity incorporation (phospholipids +52.0%, gangliosides +193.1%) in cells fed with tritiated palmitic acid. No change with respect to cholesterol was observed. Strikingly, under these conditions, the ubiquitination state of cell proteins strongly increased. These effects were not observed with the (35-25) reverse sequence peptide. Similarly to Aβ, lactacystin treatment increased lipid synthesis and protein ubiquitination; only lactacystin, and not Aβ, induced a strong decrease of proteasome chimotrypsin activity. These results suggest that Aβ enhances protein ubiquitination, without inhibiting proteasomal activity, and lipid synthesis. These results may shed new light on the mechanisms of Aβ toxicity.
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U2 - 10.1002/jnr.21354
DO - 10.1002/jnr.21354
M3 - Article
C2 - 17510978
AN - SCOPUS:34547452202
SN - 0360-4012
VL - 85
SP - 2253
EP - 2261
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 10
ER -