γ-Tubulin-like molecules in the mouse duodenal epithelium

Etsuko Suzaki, Ryuji Nomura, Tetsuya Horio, Yoshinobu Mineyuki, Katsuko Kataoka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A mouse monoclonal antibody (G9, Horio et al. in Cell Motil Cytoskel 44:284-295, 1999) that was raised against the γ-tubulin from a fission yeast, Schizosaccharomyces pombe, showed a unique staining in the mouse small intestine. Similar to another anti-γ-tubulin antibody that is commercially available, G9 showed typical dot-like staining corresponding to the microtubule-organizing center in the free cells of the epithelium and the connective tissue under it. In addition, G9 stained the cell-cell contacts in the epithelium. This stained region was not bicellular but tricellular junctions of the enterocytes. This staining was unique to G9 and was diminished on the sample of the mouse small intestine, which had lost most of its filamentous microtubules through the preparation process. The tricellular junction is thought to be the weakest point of the epithelial barrier, and no other junctional structures have been identified except for the central sealing elements extending from the tight junctions between the two cells. Our results suggest the existence of a new molecule underlying the tricellular junctions, which may relate to γ-tubulin and the microtubules.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalHistochemistry and Cell Biology
Volume128
Issue number2
DOIs
Publication statusPublished - 01-08-2007

Fingerprint

Monoclonal antibodies
epithelium
Tubulin
Antibodies
Yeast
mice
Epithelium
Tissue
staining
Molecules
Schizosaccharomyces
Staining and Labeling
cells
intestines
Microtubules
Small Intestine
molecules
antibodies
Microtubule-Organizing Center
Connective Tissue Cells

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Instrumentation

Cite this

Suzaki, Etsuko ; Nomura, Ryuji ; Horio, Tetsuya ; Mineyuki, Yoshinobu ; Kataoka, Katsuko. / γ-Tubulin-like molecules in the mouse duodenal epithelium. In: Histochemistry and Cell Biology. 2007 ; Vol. 128, No. 2. pp. 175-182.
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γ-Tubulin-like molecules in the mouse duodenal epithelium. / Suzaki, Etsuko; Nomura, Ryuji; Horio, Tetsuya; Mineyuki, Yoshinobu; Kataoka, Katsuko.

In: Histochemistry and Cell Biology, Vol. 128, No. 2, 01.08.2007, p. 175-182.

Research output: Contribution to journalArticle

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