27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells

Yuichiro Hashimoto, Hisatoshi Sugiura, Shinsaku Togo, Akira Koarai, Kyoko Abe, Mitsuhiro Yamada, Tomohiro Ichikawa, Takashi Kikuchi, Tadahisa Numakura, Katsuhiro Onodera, Rie Tanaka, Kei Sato, Satoru Yanagisawa, Tatsuma Okazaki, Tsutomu Tamada, Toshiaki Kikuchi, Yasushi Hoshikawa, Yoshinori Okada, Masakazu Ichinose

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Abstract

Cellular senescence is reportedly involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We previously showed that 27-hydroxycholesterol (27-OHC) is elevated in the airways of COPD patients compared with those in healthy subjects. The aim of this study was to investigate whether lung fibroblasts of COPD patients are senescent and to determine the effects of 27-OHC on senescence of lung resident cells, including fibroblasts and airway epithelial cells. Localization of senescence-associated proteins and sterol 27-hydroxylase was investigated in the lungs of COPD patients by immunohistochemical staining. To evaluate whether 27-OHC accelerates cellular senescence, lung resident cells were exposed to 27-OHC. Senescence markers and fibroblast-mediated tissue repair were investigated in the 27-OHC-treated cells. Expression of senescence-associated proteins was significantly enhanced in lung fibroblasts of COPD patients. Similarly, expression of sterol 27-hydroxylase was significantly upregulated in lung fibroblasts and alveolar macrophages in these patients. Treatment with the concentration of 27-OHC detected in COPD airways significantly augmented expression of senescenceassociated proteins and senescence-associated β-galactosidase activity, and delayed cell growth through the prostaglandin E2-reactive nitrogen species pathway. The 27-OHC-treated fibroblasts impaired tissue repair function. Fibroblasts from lungs of COPD patients showed accelerated senescence and were more susceptible to 27-OHC-induced cellular senescence compared with those of healthy subjects. In conclusion, 27-OHC accelerates cellular senescence in lung resident cells and may play a pivotal role in cellular senescence in COPD.

Original languageEnglish
Pages (from-to)L1028-L1041
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume310
Issue number11
DOIs
Publication statusPublished - 01-06-2016

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Cell Aging
Chronic Obstructive Pulmonary Disease
Fibroblasts
Lung
Cholestanetriol 26-Monooxygenase
Healthy Volunteers
Galactosidases
Reactive Nitrogen Species
Proteins
27-hydroxycholesterol
Alveolar Macrophages
Dinoprostone
Epithelial Cells
Staining and Labeling
Growth

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Hashimoto, Yuichiro ; Sugiura, Hisatoshi ; Togo, Shinsaku ; Koarai, Akira ; Abe, Kyoko ; Yamada, Mitsuhiro ; Ichikawa, Tomohiro ; Kikuchi, Takashi ; Numakura, Tadahisa ; Onodera, Katsuhiro ; Tanaka, Rie ; Sato, Kei ; Yanagisawa, Satoru ; Okazaki, Tatsuma ; Tamada, Tsutomu ; Kikuchi, Toshiaki ; Hoshikawa, Yasushi ; Okada, Yoshinori ; Ichinose, Masakazu. / 27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2016 ; Vol. 310, No. 11. pp. L1028-L1041.
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title = "27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells",
abstract = "Cellular senescence is reportedly involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We previously showed that 27-hydroxycholesterol (27-OHC) is elevated in the airways of COPD patients compared with those in healthy subjects. The aim of this study was to investigate whether lung fibroblasts of COPD patients are senescent and to determine the effects of 27-OHC on senescence of lung resident cells, including fibroblasts and airway epithelial cells. Localization of senescence-associated proteins and sterol 27-hydroxylase was investigated in the lungs of COPD patients by immunohistochemical staining. To evaluate whether 27-OHC accelerates cellular senescence, lung resident cells were exposed to 27-OHC. Senescence markers and fibroblast-mediated tissue repair were investigated in the 27-OHC-treated cells. Expression of senescence-associated proteins was significantly enhanced in lung fibroblasts of COPD patients. Similarly, expression of sterol 27-hydroxylase was significantly upregulated in lung fibroblasts and alveolar macrophages in these patients. Treatment with the concentration of 27-OHC detected in COPD airways significantly augmented expression of senescenceassociated proteins and senescence-associated β-galactosidase activity, and delayed cell growth through the prostaglandin E2-reactive nitrogen species pathway. The 27-OHC-treated fibroblasts impaired tissue repair function. Fibroblasts from lungs of COPD patients showed accelerated senescence and were more susceptible to 27-OHC-induced cellular senescence compared with those of healthy subjects. In conclusion, 27-OHC accelerates cellular senescence in lung resident cells and may play a pivotal role in cellular senescence in COPD.",
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Hashimoto, Y, Sugiura, H, Togo, S, Koarai, A, Abe, K, Yamada, M, Ichikawa, T, Kikuchi, T, Numakura, T, Onodera, K, Tanaka, R, Sato, K, Yanagisawa, S, Okazaki, T, Tamada, T, Kikuchi, T, Hoshikawa, Y, Okada, Y & Ichinose, M 2016, '27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 310, no. 11, pp. L1028-L1041. https://doi.org/10.1152/ajplung.00351.2015

27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells. / Hashimoto, Yuichiro; Sugiura, Hisatoshi; Togo, Shinsaku; Koarai, Akira; Abe, Kyoko; Yamada, Mitsuhiro; Ichikawa, Tomohiro; Kikuchi, Takashi; Numakura, Tadahisa; Onodera, Katsuhiro; Tanaka, Rie; Sato, Kei; Yanagisawa, Satoru; Okazaki, Tatsuma; Tamada, Tsutomu; Kikuchi, Toshiaki; Hoshikawa, Yasushi; Okada, Yoshinori; Ichinose, Masakazu.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 310, No. 11, 01.06.2016, p. L1028-L1041.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 27-Hydroxycholesterol accelerates cellular senescence in human lung resident cells

AU - Hashimoto, Yuichiro

AU - Sugiura, Hisatoshi

AU - Togo, Shinsaku

AU - Koarai, Akira

AU - Abe, Kyoko

AU - Yamada, Mitsuhiro

AU - Ichikawa, Tomohiro

AU - Kikuchi, Takashi

AU - Numakura, Tadahisa

AU - Onodera, Katsuhiro

AU - Tanaka, Rie

AU - Sato, Kei

AU - Yanagisawa, Satoru

AU - Okazaki, Tatsuma

AU - Tamada, Tsutomu

AU - Kikuchi, Toshiaki

AU - Hoshikawa, Yasushi

AU - Okada, Yoshinori

AU - Ichinose, Masakazu

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Cellular senescence is reportedly involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We previously showed that 27-hydroxycholesterol (27-OHC) is elevated in the airways of COPD patients compared with those in healthy subjects. The aim of this study was to investigate whether lung fibroblasts of COPD patients are senescent and to determine the effects of 27-OHC on senescence of lung resident cells, including fibroblasts and airway epithelial cells. Localization of senescence-associated proteins and sterol 27-hydroxylase was investigated in the lungs of COPD patients by immunohistochemical staining. To evaluate whether 27-OHC accelerates cellular senescence, lung resident cells were exposed to 27-OHC. Senescence markers and fibroblast-mediated tissue repair were investigated in the 27-OHC-treated cells. Expression of senescence-associated proteins was significantly enhanced in lung fibroblasts of COPD patients. Similarly, expression of sterol 27-hydroxylase was significantly upregulated in lung fibroblasts and alveolar macrophages in these patients. Treatment with the concentration of 27-OHC detected in COPD airways significantly augmented expression of senescenceassociated proteins and senescence-associated β-galactosidase activity, and delayed cell growth through the prostaglandin E2-reactive nitrogen species pathway. The 27-OHC-treated fibroblasts impaired tissue repair function. Fibroblasts from lungs of COPD patients showed accelerated senescence and were more susceptible to 27-OHC-induced cellular senescence compared with those of healthy subjects. In conclusion, 27-OHC accelerates cellular senescence in lung resident cells and may play a pivotal role in cellular senescence in COPD.

AB - Cellular senescence is reportedly involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). We previously showed that 27-hydroxycholesterol (27-OHC) is elevated in the airways of COPD patients compared with those in healthy subjects. The aim of this study was to investigate whether lung fibroblasts of COPD patients are senescent and to determine the effects of 27-OHC on senescence of lung resident cells, including fibroblasts and airway epithelial cells. Localization of senescence-associated proteins and sterol 27-hydroxylase was investigated in the lungs of COPD patients by immunohistochemical staining. To evaluate whether 27-OHC accelerates cellular senescence, lung resident cells were exposed to 27-OHC. Senescence markers and fibroblast-mediated tissue repair were investigated in the 27-OHC-treated cells. Expression of senescence-associated proteins was significantly enhanced in lung fibroblasts of COPD patients. Similarly, expression of sterol 27-hydroxylase was significantly upregulated in lung fibroblasts and alveolar macrophages in these patients. Treatment with the concentration of 27-OHC detected in COPD airways significantly augmented expression of senescenceassociated proteins and senescence-associated β-galactosidase activity, and delayed cell growth through the prostaglandin E2-reactive nitrogen species pathway. The 27-OHC-treated fibroblasts impaired tissue repair function. Fibroblasts from lungs of COPD patients showed accelerated senescence and were more susceptible to 27-OHC-induced cellular senescence compared with those of healthy subjects. In conclusion, 27-OHC accelerates cellular senescence in lung resident cells and may play a pivotal role in cellular senescence in COPD.

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