3, 4-Dihydroxybenzaldehyde derived from Prunus mume seed inhibits oxidative stress and enhances estradiol secretion in human ovarian granulosa tumor cells

Ryohei Kono, Sachiko Nomura, Yoshiharu Okuno, Misa Nakamura, Akihiro Maeno, Tomoko Kagiya, Akihiko Tokuda, Ken Ichi Inada, Akira Matsuno, Tomoko Utsunomiya, Hirotoshi Utsunomiya

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects granulosa cells from hydrogen peroxide (H2O2)-induced apoptosis. We detected the bioactive compound, 3, 4-dihydroxybenzaldehyde (3, 4-DHBA), via bioactivity-guided isolation and found that it inhibited the H2O2-induced apoptosis of granulosa cells. We also showed that 3, 4-DHBA promoted estradiol secretion in granulosa cells and enhanced the mRNA expression levels of steroidogenic factor 1, a promoter of key steroidogenic enzymes. These results suggest that P. mume seed extract may have clinical potential for the prevention and treatment of female infertility.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalActa Histochemica et Cytochemica
Volume47
Issue number3
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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