TY - JOUR
T1 - 3, 4-Dihydroxybenzaldehyde derived from Prunus mume seed inhibits oxidative stress and enhances estradiol secretion in human ovarian granulosa tumor cells
AU - Kono, Ryohei
AU - Nomura, Sachiko
AU - Okuno, Yoshiharu
AU - Nakamura, Misa
AU - Maeno, Akihiro
AU - Kagiya, Tomoko
AU - Tokuda, Akihiko
AU - Inada, Ken Ichi
AU - Matsuno, Akira
AU - Utsunomiya, Tomoko
AU - Utsunomiya, Hirotoshi
PY - 2014
Y1 - 2014
N2 - Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects granulosa cells from hydrogen peroxide (H2O2)-induced apoptosis. We detected the bioactive compound, 3, 4-dihydroxybenzaldehyde (3, 4-DHBA), via bioactivity-guided isolation and found that it inhibited the H2O2-induced apoptosis of granulosa cells. We also showed that 3, 4-DHBA promoted estradiol secretion in granulosa cells and enhanced the mRNA expression levels of steroidogenic factor 1, a promoter of key steroidogenic enzymes. These results suggest that P. mume seed extract may have clinical potential for the prevention and treatment of female infertility.
AB - Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects granulosa cells from hydrogen peroxide (H2O2)-induced apoptosis. We detected the bioactive compound, 3, 4-dihydroxybenzaldehyde (3, 4-DHBA), via bioactivity-guided isolation and found that it inhibited the H2O2-induced apoptosis of granulosa cells. We also showed that 3, 4-DHBA promoted estradiol secretion in granulosa cells and enhanced the mRNA expression levels of steroidogenic factor 1, a promoter of key steroidogenic enzymes. These results suggest that P. mume seed extract may have clinical potential for the prevention and treatment of female infertility.
UR - http://www.scopus.com/inward/record.url?scp=84903612874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903612874&partnerID=8YFLogxK
U2 - 10.1267/ahc.14003
DO - 10.1267/ahc.14003
M3 - Article
AN - SCOPUS:84903612874
SN - 0044-5991
VL - 47
SP - 103
EP - 112
JO - Acta Histochemica et Cytochemica
JF - Acta Histochemica et Cytochemica
IS - 3
ER -