TY - JOUR
T1 - A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length
AU - Morita, Kiyokazu
AU - Flemming, Anthony J.
AU - Sugihara, Yukiko
AU - Mochii, Makoto
AU - Suzuki, Yo
AU - Yoshida, Satoru
AU - Wood, William B.
AU - Kohara, Yuji
AU - Leroi, Armand M.
AU - Ueno, Naoto
PY - 2002/3/1
Y1 - 2002/3/1
N2 - Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-β signaling. Worms overexpressing dbl-1, a TGF-β ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length, lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-β growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.
AB - Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-β signaling. Worms overexpressing dbl-1, a TGF-β ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length, lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-β growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.
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U2 - 10.1093/emboj/21.5.1063
DO - 10.1093/emboj/21.5.1063
M3 - Article
C2 - 11867534
AN - SCOPUS:18344380428
VL - 21
SP - 1063
EP - 1073
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 5
ER -