A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length

Kiyokazu Morita; Anthony J. Flemming; Yukiko Sugihara; Makoto Mochii; Yo Suzuki; Satoru Yoshida; William B. Wood; Yuji Kohara; Armand M. Leroi; Naoto Ueno

doi:10.1093/emboj/21.5.1063

A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length

Kiyokazu Morita, Anthony J. Flemming, Yukiko Sugihara, Makoto Mochii, Yo Suzuki, Satoru Yoshida, William B. Wood, Yuji Kohara, Armand M. Leroi, Naoto Ueno

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-β signaling. Worms overexpressing dbl-1, a TGF-β ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length, lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-β growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.

Original language	English
Pages (from-to)	1063-1073
Number of pages	11
Journal	EMBO Journal
Volume	21
Issue number	5
DOIs	https://doi.org/10.1093/emboj/21.5.1063
Publication status	Published - 01-03-2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1093/emboj/21.5.1063

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Morita, Kiyokazu ; Flemming, Anthony J. ; Sugihara, Yukiko ; Mochii, Makoto ; Suzuki, Yo ; Yoshida, Satoru ; Wood, William B. ; Kohara, Yuji ; Leroi, Armand M. ; Ueno, Naoto. / A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length. In: EMBO Journal. 2002 ; Vol. 21, No. 5. pp. 1063-1073.

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title = "A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length",

abstract = "Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-β signaling. Worms overexpressing dbl-1, a TGF-β ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length, lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-β growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.",

author = "Kiyokazu Morita and Flemming, {Anthony J.} and Yukiko Sugihara and Makoto Mochii and Yo Suzuki and Satoru Yoshida and Wood, {William B.} and Yuji Kohara and Leroi, {Armand M.} and Naoto Ueno",

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A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length. / Morita, Kiyokazu; Flemming, Anthony J.; Sugihara, Yukiko; Mochii, Makoto; Suzuki, Yo; Yoshida, Satoru; Wood, William B.; Kohara, Yuji; Leroi, Armand M.; Ueno, Naoto.

In: EMBO Journal, Vol. 21, No. 5, 01.03.2002, p. 1063-1073.

Research output: Contribution to journal › Article › peer-review

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T1 - A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length

AU - Morita, Kiyokazu

AU - Flemming, Anthony J.

AU - Sugihara, Yukiko

AU - Mochii, Makoto

AU - Suzuki, Yo

AU - Yoshida, Satoru

AU - Wood, William B.

AU - Kohara, Yuji

AU - Leroi, Armand M.

AU - Ueno, Naoto

PY - 2002/3/1

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N2 - Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-β signaling. Worms overexpressing dbl-1, a TGF-β ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length, lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-β growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.

AB - Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-β signaling. Worms overexpressing dbl-1, a TGF-β ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length, lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-β growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.

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A caenorhabditis elegans TGF-β, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length

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