TY - JOUR
T1 - A clinical trial for therapeutic drug monitoring using microchip-based fluorescence polarization immunoassay
AU - Tachi, Tomoya
AU - Hase, Tetsunari
AU - Okamoto, Yukihiro
AU - Kaji, Noritada
AU - Arima, Takeshi
AU - Matsumoto, Hiroyuki
AU - Kondo, Masashi
AU - Tokeshi, Manabu
AU - Hasegawa, Yoshinori
AU - Baba, Yoshinobu
N1 - Funding Information:
Acknowledgments This work was partially supported by Japan Society for the Promotion Science (JSPS) through its “Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)” and the Grant-in-Aid for Scientific Research (B) (17310087). We thank Mr. Kenji Uchiyama and Mr. Yoshikuni Kikutani of the Institute of Microchemical Technology, Co., Ltd. for valuable discussions on chip fabrication.
PY - 2011/10
Y1 - 2011/10
N2 - Microchip analysis is a promising method for therapeutic drug monitoring. This led us to evaluate a microchip-based fluorescence polarization immunoassay (FPIA) system for point-of-care testing on patients being treated with theophylline. The sera were collected from 20 patients being treated with theophylline. Fluorescence polarization was measured on the microchip and theophylline concentrations in serum were obtained. Regression analysis of the correlations was done between the results given by the microchip-based FPIA and the conventional cloned enzyme donor immunoassay (CEDIA), and between the results given by the microchip-based FPIA and the conventional particle-enhanced turbidimetric inhibition immunoassay (PETINIA). We successfully carried out a quantitative analysis of theophylline in serum at values near its therapeutic range in 65 s. The results obtained by the microchip-based FPIA correlated well with CEDIA and PETINIA results; the correlation coefficients (R 2) were 0.986 and 0.989, respectively. The FPIA system is a simple and rapid method for point-of-care testing of drugs in serum, and its accuracy is the same as the conventional CEDIA and PETINIA. It is essential to use real samples from patients and to confirm good correlations with conventional methods for a study on the realization of microchip.
AB - Microchip analysis is a promising method for therapeutic drug monitoring. This led us to evaluate a microchip-based fluorescence polarization immunoassay (FPIA) system for point-of-care testing on patients being treated with theophylline. The sera were collected from 20 patients being treated with theophylline. Fluorescence polarization was measured on the microchip and theophylline concentrations in serum were obtained. Regression analysis of the correlations was done between the results given by the microchip-based FPIA and the conventional cloned enzyme donor immunoassay (CEDIA), and between the results given by the microchip-based FPIA and the conventional particle-enhanced turbidimetric inhibition immunoassay (PETINIA). We successfully carried out a quantitative analysis of theophylline in serum at values near its therapeutic range in 65 s. The results obtained by the microchip-based FPIA correlated well with CEDIA and PETINIA results; the correlation coefficients (R 2) were 0.986 and 0.989, respectively. The FPIA system is a simple and rapid method for point-of-care testing of drugs in serum, and its accuracy is the same as the conventional CEDIA and PETINIA. It is essential to use real samples from patients and to confirm good correlations with conventional methods for a study on the realization of microchip.
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U2 - 10.1007/s00216-011-5304-9
DO - 10.1007/s00216-011-5304-9
M3 - Article
C2 - 21837465
AN - SCOPUS:81155159661
SN - 1618-2642
VL - 401
SP - 2301
EP - 2305
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 7
ER -