TY - JOUR
T1 - A comparison of techniques for collecting skin microbiome samples
T2 - Swabbing versus tape-stripping
AU - Ogai, Kazuhiro
AU - Nagase, Satoshi
AU - Mukai, Kanae
AU - Iuchi, Terumi
AU - Mori, Yumiko
AU - Matsue, Miki
AU - Sugitani, Kayo
AU - Sugama, Junko
AU - Okamoto, Shigefumi
N1 - Funding Information:
This study was supported in part by Kanazawa University SAKIGAKE Project and by the Ministry of Education, Culture, Sports, Science and Technology/Japan Science
Publisher Copyright:
© 2018 Ogai, Nagase, Mukai, Iuchi, Mori, Matsue, Sugitani, Sugama and Okamoto.
PY - 2018/10/2
Y1 - 2018/10/2
N2 - The swabbing and tape-stripping methods have traditionally been used for collecting skin microbiome samples for skin bacterial analysis, although no reports have compared the outcome of these methods for collecting skin bacteria. Our purpose was to show the differences in microbial composition between samples collected using the swabbing and tape-stripping methods, by both the next generation sequencing and culture studies. The skin microbiome was collected by both methods, and the samples were processed for a sequence-based microbiome analysis and culture study. The next-generation sequencing results showed that skin bacteria collected using the tape-stripping method were comparable to those collected using the swabbing method. In the culture study, the tape-stripping method collected a greater number and wider variety of viable skin bacteria than the swabbing method. These results suggest that the tape-stripping method is comparable to the swabbing method for collecting viable skin bacteria, without losing fidelity to the composition of skin microbiome.
AB - The swabbing and tape-stripping methods have traditionally been used for collecting skin microbiome samples for skin bacterial analysis, although no reports have compared the outcome of these methods for collecting skin bacteria. Our purpose was to show the differences in microbial composition between samples collected using the swabbing and tape-stripping methods, by both the next generation sequencing and culture studies. The skin microbiome was collected by both methods, and the samples were processed for a sequence-based microbiome analysis and culture study. The next-generation sequencing results showed that skin bacteria collected using the tape-stripping method were comparable to those collected using the swabbing method. In the culture study, the tape-stripping method collected a greater number and wider variety of viable skin bacteria than the swabbing method. These results suggest that the tape-stripping method is comparable to the swabbing method for collecting viable skin bacteria, without losing fidelity to the composition of skin microbiome.
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U2 - 10.3389/fmicb.2018.02362
DO - 10.3389/fmicb.2018.02362
M3 - Article
AN - SCOPUS:85055179761
VL - 9
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - OCT
M1 - 2362
ER -