A cyclosporin A/maltosyl-α-cyclosdextrin complex for inhalation therapy of asthma

Hiroaki Fukaya, A. Iimura, K. Hoshiko, T. Fuyumuro, S. Noji, Toshitaka Nabeshima

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The inhalation of cyclosporin (Cs)A to the lung is limited by its hydrophobic properties. In order to improve the poor solubility of CsA, cyclodextrin (CD) was evaluated for its suitability for dry powder inhaler formation, and the benefit of an inhaled CsA/CD complex in vivo was demonstrated. The solubilising effect of CDs on CsA was measured by high-performance liquid chromatography. Ciliostatic activity and haemolysis were determined to assess some safety profiles of CDs. The efficacy of an inhaled CsA/CD complex was evaluated by eosinophil infiltration into the bronchoalveolar lavage fluid in actively sensitised mice. CDs markedly improved the poor solubility of CsA. The ciliostatic and haemolytic activities of maltosyl-α-CD were the weakest of all the tested CDs. CsA inhaled alone showed inhibitory effects on allergen-induced eosinophilia. Inhalation of the complex of CsA with maltosyl-α-CD, where the dose of CsA was approximately nine-times less than that of CsA inhaled alone, also inhibited eosinophil accumulation significantly, with a longer duration of action in comparison with the response to CsA alone. Thus the effective dose of cyclosporin A could be reduced by formation of a complex with maltosyl-α-cyclodextrin, and a wider therapeutic safety margin by inhalation of cyclosporin A as a complex with maltosyl-α-cyclodextrin could be expected.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalEuropean Respiratory Journal
Volume22
Issue number2
DOIs
Publication statusPublished - 01-08-2003

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Respiratory Therapy
Cyclosporine
Cyclodextrins
Asthma
Inhalation
Eosinophils
Solubility
Dry Powder Inhalers
Safety
Bronchoalveolar Lavage Fluid
Eosinophilia
Hemolysis
Allergens
High Pressure Liquid Chromatography
Lung
maltosyl-cyclodextrin
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Fukaya, Hiroaki ; Iimura, A. ; Hoshiko, K. ; Fuyumuro, T. ; Noji, S. ; Nabeshima, Toshitaka. / A cyclosporin A/maltosyl-α-cyclosdextrin complex for inhalation therapy of asthma. In: European Respiratory Journal. 2003 ; Vol. 22, No. 2. pp. 213-219.
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A cyclosporin A/maltosyl-α-cyclosdextrin complex for inhalation therapy of asthma. / Fukaya, Hiroaki; Iimura, A.; Hoshiko, K.; Fuyumuro, T.; Noji, S.; Nabeshima, Toshitaka.

In: European Respiratory Journal, Vol. 22, No. 2, 01.08.2003, p. 213-219.

Research output: Contribution to journalArticle

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