A dried blood sample on filter paper is suitable for detecting Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction

Yoshimasa Maeno, Shusuke Nakazawa, Le Duc Dao, Naoki Yamamoto, Nguyen Duc Giang, Truong Van Hanh, Le Khanh Thuan, Koki Taniguchi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The detection of gametocytes in human peripheral blood is one of the most important measures in a malaria survey. We attempted to detect gametocytes of Plasmodium falciparum by reverse transcription polymerase chain reaction (RT-PCR) of dried blood on filter paper. On field samples analysis, the specific RT-PCR products for region 3 of pfg377 mRNA were observed in 67 of 131 falciparum malaria patients. The minimum detection level of RT-PCR-positive samples was 0.03 gametocytes/μl on quantitative real-time RT-PCR. Gametocyte positive rate was not dependent on sex or age. A higher frequency of gametocytes was found in single P. falciparum infection than in mixed species infection (P < 0.01). In this study, 47 of the 131 patients were asymptomatic. Eighteen of these 47 patients showed pfg377 mRNA expression. Moreover, four alleles of region 3 of pfg377 were detected in pfg377 mRNA-positive patients and 13 of 67 pfg377 mRNA-positive patients carried more than one gametocyte-producing clone. These results suggest that dried blood on filter paper is a useful for a molecular epidemiologic study of malaria transmission and gametocyte-targeted control.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalActa Tropica
Volume107
Issue number2
DOIs
Publication statusPublished - 01-08-2008

Fingerprint

gametocytes
Plasmodium falciparum
Reverse Transcription
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
blood
Malaria
Messenger RNA
malaria
sampling
Falciparum Malaria
Coinfection
Epidemiologic Studies
Clone Cells
Alleles
mixed infection
epidemiological studies
quantitative polymerase chain reaction
clones
alleles

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Maeno, Yoshimasa ; Nakazawa, Shusuke ; Dao, Le Duc ; Yamamoto, Naoki ; Giang, Nguyen Duc ; Van Hanh, Truong ; Thuan, Le Khanh ; Taniguchi, Koki. / A dried blood sample on filter paper is suitable for detecting Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction. In: Acta Tropica. 2008 ; Vol. 107, No. 2. pp. 121-127.
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A dried blood sample on filter paper is suitable for detecting Plasmodium falciparum gametocytes by reverse transcription polymerase chain reaction. / Maeno, Yoshimasa; Nakazawa, Shusuke; Dao, Le Duc; Yamamoto, Naoki; Giang, Nguyen Duc; Van Hanh, Truong; Thuan, Le Khanh; Taniguchi, Koki.

In: Acta Tropica, Vol. 107, No. 2, 01.08.2008, p. 121-127.

Research output: Contribution to journalArticle

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