A F240S polymorphism of protease-activated receptor 2 (PAR2) is not detected in Japanese population with gastro-esophageal symptoms

Tomiyasu Arisawa, Tomomitsu Tahara, Tomoyuki Shibata, Mitsuo Nagasaka, Masakatsu Nakamura, Yoshio Kamiya, Hiroshi Fujita, Shin Hasegawa, Masahiko Nakamura, Tamaki Takagi, Ichiro Hirata, Hiroshi Nakano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Trypsin acting at protease-activated receptor 2 (PAR2) has been reported to contribute to a progression of malignant tumors. In addition, a polymorphic form of PAR2 has been also reported to display reduced sensitivity to trypsin. However, a frequency of PAR2 polymorphism is unknown in Japan. The aim of the present study was to clarify a frequency of PAR2 polymorphism in Japan and to evaluate the relation between this polymorphism and gastric cancer. We estimated PAR2 T719C in 106 patients with gastric cancer (GC cases) and 96 patients without gastric cancer (non-GC cases). We employed a single-strand conformation polymorphism analysis after polymerase chain reaction (PCR-SSCP) for detecting of this polymorphism. There was no significant difference between GC and non-GC cases in the distribution of gender (M:F = 2.66 and 2.00, respectively) and age (mean age = 66.12 and 63.50, respectively). Helicobacter pylori (H. pylori) positive ratio was 81.7% (GC cases: 92.0%, non-GC cases: 72.5%, p<.01). Single strand bands of 719C were not detected in all 202 patients by PCR-SSCP. In conclusion, a polymorphism F240S of PAR2 is very rare and does not contribute to the genesis and progression of gastric cancer in Japanese population.

Original languageEnglish
Pages (from-to)98-101
Number of pages4
JournalJournal of Clinical Biochemistry and Nutrition
Volume39
Issue number2
DOIs
Publication statusPublished - 01-09-2006

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PAR-2 Receptor
Polymorphism
Stomach Neoplasms
Population
Single-Stranded Conformational Polymorphism
Polymerase Chain Reaction
Trypsin
Japan
Polymerase chain reaction
Helicobacter pylori
Conformations
Tumors

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Arisawa, Tomiyasu ; Tahara, Tomomitsu ; Shibata, Tomoyuki ; Nagasaka, Mitsuo ; Nakamura, Masakatsu ; Kamiya, Yoshio ; Fujita, Hiroshi ; Hasegawa, Shin ; Nakamura, Masahiko ; Takagi, Tamaki ; Hirata, Ichiro ; Nakano, Hiroshi. / A F240S polymorphism of protease-activated receptor 2 (PAR2) is not detected in Japanese population with gastro-esophageal symptoms. In: Journal of Clinical Biochemistry and Nutrition. 2006 ; Vol. 39, No. 2. pp. 98-101.
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title = "A F240S polymorphism of protease-activated receptor 2 (PAR2) is not detected in Japanese population with gastro-esophageal symptoms",
abstract = "Trypsin acting at protease-activated receptor 2 (PAR2) has been reported to contribute to a progression of malignant tumors. In addition, a polymorphic form of PAR2 has been also reported to display reduced sensitivity to trypsin. However, a frequency of PAR2 polymorphism is unknown in Japan. The aim of the present study was to clarify a frequency of PAR2 polymorphism in Japan and to evaluate the relation between this polymorphism and gastric cancer. We estimated PAR2 T719C in 106 patients with gastric cancer (GC cases) and 96 patients without gastric cancer (non-GC cases). We employed a single-strand conformation polymorphism analysis after polymerase chain reaction (PCR-SSCP) for detecting of this polymorphism. There was no significant difference between GC and non-GC cases in the distribution of gender (M:F = 2.66 and 2.00, respectively) and age (mean age = 66.12 and 63.50, respectively). Helicobacter pylori (H. pylori) positive ratio was 81.7{\%} (GC cases: 92.0{\%}, non-GC cases: 72.5{\%}, p<.01). Single strand bands of 719C were not detected in all 202 patients by PCR-SSCP. In conclusion, a polymorphism F240S of PAR2 is very rare and does not contribute to the genesis and progression of gastric cancer in Japanese population.",
author = "Tomiyasu Arisawa and Tomomitsu Tahara and Tomoyuki Shibata and Mitsuo Nagasaka and Masakatsu Nakamura and Yoshio Kamiya and Hiroshi Fujita and Shin Hasegawa and Masahiko Nakamura and Tamaki Takagi and Ichiro Hirata and Hiroshi Nakano",
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Arisawa, T, Tahara, T, Shibata, T, Nagasaka, M, Nakamura, M, Kamiya, Y, Fujita, H, Hasegawa, S, Nakamura, M, Takagi, T, Hirata, I & Nakano, H 2006, 'A F240S polymorphism of protease-activated receptor 2 (PAR2) is not detected in Japanese population with gastro-esophageal symptoms', Journal of Clinical Biochemistry and Nutrition, vol. 39, no. 2, pp. 98-101. https://doi.org/10.3164/jcbn.39.98

A F240S polymorphism of protease-activated receptor 2 (PAR2) is not detected in Japanese population with gastro-esophageal symptoms. / Arisawa, Tomiyasu; Tahara, Tomomitsu; Shibata, Tomoyuki; Nagasaka, Mitsuo; Nakamura, Masakatsu; Kamiya, Yoshio; Fujita, Hiroshi; Hasegawa, Shin; Nakamura, Masahiko; Takagi, Tamaki; Hirata, Ichiro; Nakano, Hiroshi.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 39, No. 2, 01.09.2006, p. 98-101.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A F240S polymorphism of protease-activated receptor 2 (PAR2) is not detected in Japanese population with gastro-esophageal symptoms

AU - Arisawa, Tomiyasu

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Nagasaka, Mitsuo

AU - Nakamura, Masakatsu

AU - Kamiya, Yoshio

AU - Fujita, Hiroshi

AU - Hasegawa, Shin

AU - Nakamura, Masahiko

AU - Takagi, Tamaki

AU - Hirata, Ichiro

AU - Nakano, Hiroshi

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Trypsin acting at protease-activated receptor 2 (PAR2) has been reported to contribute to a progression of malignant tumors. In addition, a polymorphic form of PAR2 has been also reported to display reduced sensitivity to trypsin. However, a frequency of PAR2 polymorphism is unknown in Japan. The aim of the present study was to clarify a frequency of PAR2 polymorphism in Japan and to evaluate the relation between this polymorphism and gastric cancer. We estimated PAR2 T719C in 106 patients with gastric cancer (GC cases) and 96 patients without gastric cancer (non-GC cases). We employed a single-strand conformation polymorphism analysis after polymerase chain reaction (PCR-SSCP) for detecting of this polymorphism. There was no significant difference between GC and non-GC cases in the distribution of gender (M:F = 2.66 and 2.00, respectively) and age (mean age = 66.12 and 63.50, respectively). Helicobacter pylori (H. pylori) positive ratio was 81.7% (GC cases: 92.0%, non-GC cases: 72.5%, p<.01). Single strand bands of 719C were not detected in all 202 patients by PCR-SSCP. In conclusion, a polymorphism F240S of PAR2 is very rare and does not contribute to the genesis and progression of gastric cancer in Japanese population.

AB - Trypsin acting at protease-activated receptor 2 (PAR2) has been reported to contribute to a progression of malignant tumors. In addition, a polymorphic form of PAR2 has been also reported to display reduced sensitivity to trypsin. However, a frequency of PAR2 polymorphism is unknown in Japan. The aim of the present study was to clarify a frequency of PAR2 polymorphism in Japan and to evaluate the relation between this polymorphism and gastric cancer. We estimated PAR2 T719C in 106 patients with gastric cancer (GC cases) and 96 patients without gastric cancer (non-GC cases). We employed a single-strand conformation polymorphism analysis after polymerase chain reaction (PCR-SSCP) for detecting of this polymorphism. There was no significant difference between GC and non-GC cases in the distribution of gender (M:F = 2.66 and 2.00, respectively) and age (mean age = 66.12 and 63.50, respectively). Helicobacter pylori (H. pylori) positive ratio was 81.7% (GC cases: 92.0%, non-GC cases: 72.5%, p<.01). Single strand bands of 719C were not detected in all 202 patients by PCR-SSCP. In conclusion, a polymorphism F240S of PAR2 is very rare and does not contribute to the genesis and progression of gastric cancer in Japanese population.

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