A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

Wei Wang; Takanori Uzawa; Naoya Tochio; Jumpei Hamatsu; Yoshinori Hirano; Seiichi Tada; Hisao Saneyoshi; Takanori Kigawa; Nobuhiro Hayashi; Yutaka Ito; Makoto Taiji; Toshiro Aigaki; Yoshihiro Ito

doi:10.1039/c3cc47624c

A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

Wei Wang, Takanori Uzawa, Naoya Tochio, Jumpei Hamatsu, Yoshinori Hirano, Seiichi Tada, Hisao Saneyoshi, Takanori Kigawa, Nobuhiro Hayashi, Yutaka Ito, Makoto Taiji, Toshiro Aigaki, Yoshihiro Ito

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.

Original language	English
Pages (from-to)	2962-2964
Number of pages	3
Journal	Chemical Communications
Volume	50
Issue number	22
DOIs	https://doi.org/10.1039/c3cc47624c
Publication status	Published - 18-02-2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c3cc47624c

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abstract = "A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.",

author = "Wei Wang and Takanori Uzawa and Naoya Tochio and Jumpei Hamatsu and Yoshinori Hirano and Seiichi Tada and Hisao Saneyoshi and Takanori Kigawa and Nobuhiro Hayashi and Yutaka Ito and Makoto Taiji and Toshiro Aigaki and Yoshihiro Ito",

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T1 - A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

AU - Wang, Wei

AU - Uzawa, Takanori

AU - Tochio, Naoya

AU - Hamatsu, Jumpei

AU - Hirano, Yoshinori

AU - Tada, Seiichi

AU - Saneyoshi, Hisao

AU - Kigawa, Takanori

AU - Hayashi, Nobuhiro

AU - Ito, Yutaka

AU - Taiji, Makoto

AU - Aigaki, Toshiro

AU - Ito, Yoshihiro

PY - 2014/2/18

Y1 - 2014/2/18

N2 - A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.

AB - A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.

UR - https://www.scopus.com/pages/publications/84894419284

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U2 - 10.1039/c3cc47624c

DO - 10.1039/c3cc47624c

M3 - Article

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AN - SCOPUS:84894419284

SN - 1359-7345

VL - 50

SP - 2962

EP - 2964

JO - Chemical Communications

JF - Chemical Communications

IS - 22

ER -

A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

Abstract

All Science Journal Classification (ASJC) codes

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