A functional variation in the hypocretin neuropeptide precursor gene may be associated with obstructive sleep apnea syndrome in Japan

Wael A. Ahmed, Makiko Tsutsumi, Seiichi Nakata, Terumi Mori, Yoichi Nishimura, Toshiyuki Fujisawa, Ichiro Kato, Mayuki Nakashima, Hiroki Kurahashi, Kenji Suzuki

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Abstract

Objectives/Hypothesis: To evaluate the association of hypocretin neuropeptide precursor gene (HCRT) variations with obstructive sleep apnea syndrome (OSAS) in a cohort of Japanese patients and to further evaluate whether the significant HCRT variations have potential functional consequences on HCRT expression. Study Design: Case-control genetic association study. Methods: We studied the genetic variations within the HCRT gene. The study population consisted of 100 OSAS patients and 100 control subjects. The HCRT gene was amplified by polymerase chain reaction in all study subjects followed by direct sequencing and analysis of sequencing data. Results: Two genetic variations within the HCRT intron, IVS1+16T>C (rs9902709) and IVS1-69G>C, were identified with significant differences between patients and controls (P <.05). A reporter gene assay using HeLa cells showed that the construct containing the C allele of the rs9902709 variation had significantly higher luciferase activity compared with the construct containing the T allele (P =.002). Furthermore, enzyme immunoassay revealed that subjects with T/C and C/C genotypes for rs9902709 had 1.4-fold and 1.5-fold increases in sera levels of orexin-A, respectively. Conclusions: Our genetic association study, followed by functional and quantitative phenotyping assays, demonstrated a functional locus within the HCRT gene, which may act to increase HCRT expression and lead to a protective effect against the development of OSAS.

Original languageEnglish
Pages (from-to)925-929
Number of pages5
JournalLaryngoscope
Volume122
Issue number4
DOIs
Publication statusPublished - 01-04-2012

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Obstructive Sleep Apnea
Neuropeptides
Japan
Genetic Association Studies
Genes
Alleles
Luciferases
Immunoenzyme Techniques
Reporter Genes
HeLa Cells
Introns
Case-Control Studies
Genotype
Polymerase Chain Reaction
Orexins
Serum
Population

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Ahmed, Wael A. ; Tsutsumi, Makiko ; Nakata, Seiichi ; Mori, Terumi ; Nishimura, Yoichi ; Fujisawa, Toshiyuki ; Kato, Ichiro ; Nakashima, Mayuki ; Kurahashi, Hiroki ; Suzuki, Kenji. / A functional variation in the hypocretin neuropeptide precursor gene may be associated with obstructive sleep apnea syndrome in Japan. In: Laryngoscope. 2012 ; Vol. 122, No. 4. pp. 925-929.
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abstract = "Objectives/Hypothesis: To evaluate the association of hypocretin neuropeptide precursor gene (HCRT) variations with obstructive sleep apnea syndrome (OSAS) in a cohort of Japanese patients and to further evaluate whether the significant HCRT variations have potential functional consequences on HCRT expression. Study Design: Case-control genetic association study. Methods: We studied the genetic variations within the HCRT gene. The study population consisted of 100 OSAS patients and 100 control subjects. The HCRT gene was amplified by polymerase chain reaction in all study subjects followed by direct sequencing and analysis of sequencing data. Results: Two genetic variations within the HCRT intron, IVS1+16T>C (rs9902709) and IVS1-69G>C, were identified with significant differences between patients and controls (P <.05). A reporter gene assay using HeLa cells showed that the construct containing the C allele of the rs9902709 variation had significantly higher luciferase activity compared with the construct containing the T allele (P =.002). Furthermore, enzyme immunoassay revealed that subjects with T/C and C/C genotypes for rs9902709 had 1.4-fold and 1.5-fold increases in sera levels of orexin-A, respectively. Conclusions: Our genetic association study, followed by functional and quantitative phenotyping assays, demonstrated a functional locus within the HCRT gene, which may act to increase HCRT expression and lead to a protective effect against the development of OSAS.",
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A functional variation in the hypocretin neuropeptide precursor gene may be associated with obstructive sleep apnea syndrome in Japan. / Ahmed, Wael A.; Tsutsumi, Makiko; Nakata, Seiichi; Mori, Terumi; Nishimura, Yoichi; Fujisawa, Toshiyuki; Kato, Ichiro; Nakashima, Mayuki; Kurahashi, Hiroki; Suzuki, Kenji.

In: Laryngoscope, Vol. 122, No. 4, 01.04.2012, p. 925-929.

Research output: Contribution to journalArticle

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AU - Ahmed, Wael A.

AU - Tsutsumi, Makiko

AU - Nakata, Seiichi

AU - Mori, Terumi

AU - Nishimura, Yoichi

AU - Fujisawa, Toshiyuki

AU - Kato, Ichiro

AU - Nakashima, Mayuki

AU - Kurahashi, Hiroki

AU - Suzuki, Kenji

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N2 - Objectives/Hypothesis: To evaluate the association of hypocretin neuropeptide precursor gene (HCRT) variations with obstructive sleep apnea syndrome (OSAS) in a cohort of Japanese patients and to further evaluate whether the significant HCRT variations have potential functional consequences on HCRT expression. Study Design: Case-control genetic association study. Methods: We studied the genetic variations within the HCRT gene. The study population consisted of 100 OSAS patients and 100 control subjects. The HCRT gene was amplified by polymerase chain reaction in all study subjects followed by direct sequencing and analysis of sequencing data. Results: Two genetic variations within the HCRT intron, IVS1+16T>C (rs9902709) and IVS1-69G>C, were identified with significant differences between patients and controls (P <.05). A reporter gene assay using HeLa cells showed that the construct containing the C allele of the rs9902709 variation had significantly higher luciferase activity compared with the construct containing the T allele (P =.002). Furthermore, enzyme immunoassay revealed that subjects with T/C and C/C genotypes for rs9902709 had 1.4-fold and 1.5-fold increases in sera levels of orexin-A, respectively. Conclusions: Our genetic association study, followed by functional and quantitative phenotyping assays, demonstrated a functional locus within the HCRT gene, which may act to increase HCRT expression and lead to a protective effect against the development of OSAS.

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