A genetic variant of the CD14 C-159T in patients with functional dyspepsia (FD) in Japanese subjects

Tomomitsu Tahara, Tomiyasu Arisawa, Tomoyuki Shibata, Masakatsu Nakamura, Fangyu Wang, Daisuke Yoshioka, Masaaki Okubo, Naoko Maruyama, Yoshio Kamiya, Masahiko Nakamura, Hiroshi Fujita, Mitsuo Nagasaka, Masami Iwata, Kazuya Takahama, Makoto Watanabe, Ichiro Hirata, Hiroshi Nakano

Research output: Contribution to journalArticle

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Abstract

Inflammatory changes in the gastric mucosa are commonly observed in Japanese patients with functional dyspepsia (FD). However, detailed data regarding the relationship between the genetic regulatory factors of inflammation and FD are not available. CD14 is an important mediator of the inflammatory response in the first line of host defense by recognition of Lipopolysaccharide (LPS). We aimed to investigate the association between CD14 promoter C-159T polymorphism and FD in a Japanese population. 108 patients with FD and 99 non-dyspeptic subjects enrolled in this study. Dyspeptic symptoms were divided according to Rome III criteria. CD14 gene C-159T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism. In the non-dyspeptics, the CD14 genotype distribution was 28CC (28.3%), 51CT (51.5%), 21TT (21.2%). Meanwhile, the CD14 genotype distribution in FD was 31CC (28.4%), 56CT (51.4%), 22TT (20.2%). The genotype distribution was not significantly different. There was no significant difference between two groups in the genotype distribution. We did not found any association between CD14 genotypes and dyspeptic patients in different gender and Helicobacter pylori infection status. No significant association was also found between CD14 polymorphism and any of different subtypes of FD according to Rome 111 while there was a weak correlation between TT genotype and PDS in male subjects (TT vs others, OR = 3.18, 95% CI = 0.98-10.26, p = 0.06). In conclusion, our results suggest that CD14 polymorphism is unlikely to associate with susceptibility of dyspeptic symptoms. The role of inflammation related-gene polymorphisms to the development of dyspepsia needs to further evaluation.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Volume42
Issue number2
DOIs
Publication statusPublished - 01-03-2008

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Dyspepsia
Polymorphism
Genotype
Genes
Inflammation
Polymerase chain reaction
Helicobacter Infections
Lipopolysaccharides
Gastric Mucosa
Helicobacter pylori
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Population

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Tahara, Tomomitsu ; Arisawa, Tomiyasu ; Shibata, Tomoyuki ; Nakamura, Masakatsu ; Wang, Fangyu ; Yoshioka, Daisuke ; Okubo, Masaaki ; Maruyama, Naoko ; Kamiya, Yoshio ; Nakamura, Masahiko ; Fujita, Hiroshi ; Nagasaka, Mitsuo ; Iwata, Masami ; Takahama, Kazuya ; Watanabe, Makoto ; Hirata, Ichiro ; Nakano, Hiroshi. / A genetic variant of the CD14 C-159T in patients with functional dyspepsia (FD) in Japanese subjects. In: Journal of Clinical Biochemistry and Nutrition. 2008 ; Vol. 42, No. 2. pp. 104-110.
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title = "A genetic variant of the CD14 C-159T in patients with functional dyspepsia (FD) in Japanese subjects",
abstract = "Inflammatory changes in the gastric mucosa are commonly observed in Japanese patients with functional dyspepsia (FD). However, detailed data regarding the relationship between the genetic regulatory factors of inflammation and FD are not available. CD14 is an important mediator of the inflammatory response in the first line of host defense by recognition of Lipopolysaccharide (LPS). We aimed to investigate the association between CD14 promoter C-159T polymorphism and FD in a Japanese population. 108 patients with FD and 99 non-dyspeptic subjects enrolled in this study. Dyspeptic symptoms were divided according to Rome III criteria. CD14 gene C-159T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism. In the non-dyspeptics, the CD14 genotype distribution was 28CC (28.3{\%}), 51CT (51.5{\%}), 21TT (21.2{\%}). Meanwhile, the CD14 genotype distribution in FD was 31CC (28.4{\%}), 56CT (51.4{\%}), 22TT (20.2{\%}). The genotype distribution was not significantly different. There was no significant difference between two groups in the genotype distribution. We did not found any association between CD14 genotypes and dyspeptic patients in different gender and Helicobacter pylori infection status. No significant association was also found between CD14 polymorphism and any of different subtypes of FD according to Rome 111 while there was a weak correlation between TT genotype and PDS in male subjects (TT vs others, OR = 3.18, 95{\%} CI = 0.98-10.26, p = 0.06). In conclusion, our results suggest that CD14 polymorphism is unlikely to associate with susceptibility of dyspeptic symptoms. The role of inflammation related-gene polymorphisms to the development of dyspepsia needs to further evaluation.",
author = "Tomomitsu Tahara and Tomiyasu Arisawa and Tomoyuki Shibata and Masakatsu Nakamura and Fangyu Wang and Daisuke Yoshioka and Masaaki Okubo and Naoko Maruyama and Yoshio Kamiya and Masahiko Nakamura and Hiroshi Fujita and Mitsuo Nagasaka and Masami Iwata and Kazuya Takahama and Makoto Watanabe and Ichiro Hirata and Hiroshi Nakano",
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Tahara, T, Arisawa, T, Shibata, T, Nakamura, M, Wang, F, Yoshioka, D, Okubo, M, Maruyama, N, Kamiya, Y, Nakamura, M, Fujita, H, Nagasaka, M, Iwata, M, Takahama, K, Watanabe, M, Hirata, I & Nakano, H 2008, 'A genetic variant of the CD14 C-159T in patients with functional dyspepsia (FD) in Japanese subjects', Journal of Clinical Biochemistry and Nutrition, vol. 42, no. 2, pp. 104-110. https://doi.org/10.3164/jcbn.2008015

A genetic variant of the CD14 C-159T in patients with functional dyspepsia (FD) in Japanese subjects. / Tahara, Tomomitsu; Arisawa, Tomiyasu; Shibata, Tomoyuki; Nakamura, Masakatsu; Wang, Fangyu; Yoshioka, Daisuke; Okubo, Masaaki; Maruyama, Naoko; Kamiya, Yoshio; Nakamura, Masahiko; Fujita, Hiroshi; Nagasaka, Mitsuo; Iwata, Masami; Takahama, Kazuya; Watanabe, Makoto; Hirata, Ichiro; Nakano, Hiroshi.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 42, No. 2, 01.03.2008, p. 104-110.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A genetic variant of the CD14 C-159T in patients with functional dyspepsia (FD) in Japanese subjects

AU - Tahara, Tomomitsu

AU - Arisawa, Tomiyasu

AU - Shibata, Tomoyuki

AU - Nakamura, Masakatsu

AU - Wang, Fangyu

AU - Yoshioka, Daisuke

AU - Okubo, Masaaki

AU - Maruyama, Naoko

AU - Kamiya, Yoshio

AU - Nakamura, Masahiko

AU - Fujita, Hiroshi

AU - Nagasaka, Mitsuo

AU - Iwata, Masami

AU - Takahama, Kazuya

AU - Watanabe, Makoto

AU - Hirata, Ichiro

AU - Nakano, Hiroshi

PY - 2008/3/1

Y1 - 2008/3/1

N2 - Inflammatory changes in the gastric mucosa are commonly observed in Japanese patients with functional dyspepsia (FD). However, detailed data regarding the relationship between the genetic regulatory factors of inflammation and FD are not available. CD14 is an important mediator of the inflammatory response in the first line of host defense by recognition of Lipopolysaccharide (LPS). We aimed to investigate the association between CD14 promoter C-159T polymorphism and FD in a Japanese population. 108 patients with FD and 99 non-dyspeptic subjects enrolled in this study. Dyspeptic symptoms were divided according to Rome III criteria. CD14 gene C-159T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism. In the non-dyspeptics, the CD14 genotype distribution was 28CC (28.3%), 51CT (51.5%), 21TT (21.2%). Meanwhile, the CD14 genotype distribution in FD was 31CC (28.4%), 56CT (51.4%), 22TT (20.2%). The genotype distribution was not significantly different. There was no significant difference between two groups in the genotype distribution. We did not found any association between CD14 genotypes and dyspeptic patients in different gender and Helicobacter pylori infection status. No significant association was also found between CD14 polymorphism and any of different subtypes of FD according to Rome 111 while there was a weak correlation between TT genotype and PDS in male subjects (TT vs others, OR = 3.18, 95% CI = 0.98-10.26, p = 0.06). In conclusion, our results suggest that CD14 polymorphism is unlikely to associate with susceptibility of dyspeptic symptoms. The role of inflammation related-gene polymorphisms to the development of dyspepsia needs to further evaluation.

AB - Inflammatory changes in the gastric mucosa are commonly observed in Japanese patients with functional dyspepsia (FD). However, detailed data regarding the relationship between the genetic regulatory factors of inflammation and FD are not available. CD14 is an important mediator of the inflammatory response in the first line of host defense by recognition of Lipopolysaccharide (LPS). We aimed to investigate the association between CD14 promoter C-159T polymorphism and FD in a Japanese population. 108 patients with FD and 99 non-dyspeptic subjects enrolled in this study. Dyspeptic symptoms were divided according to Rome III criteria. CD14 gene C-159T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism. In the non-dyspeptics, the CD14 genotype distribution was 28CC (28.3%), 51CT (51.5%), 21TT (21.2%). Meanwhile, the CD14 genotype distribution in FD was 31CC (28.4%), 56CT (51.4%), 22TT (20.2%). The genotype distribution was not significantly different. There was no significant difference between two groups in the genotype distribution. We did not found any association between CD14 genotypes and dyspeptic patients in different gender and Helicobacter pylori infection status. No significant association was also found between CD14 polymorphism and any of different subtypes of FD according to Rome 111 while there was a weak correlation between TT genotype and PDS in male subjects (TT vs others, OR = 3.18, 95% CI = 0.98-10.26, p = 0.06). In conclusion, our results suggest that CD14 polymorphism is unlikely to associate with susceptibility of dyspeptic symptoms. The role of inflammation related-gene polymorphisms to the development of dyspepsia needs to further evaluation.

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