Abstract
β-Thalassemia/HbE disease is clinically variable. In searching for genetic factors modifying the disease severity, patients were selected based on their disease severities, and a genome-wide association study (GWAS) was performed. Genotyping was conducted with the Illumina Human 610-Quad BeadChips array using DNAs from 618 Thai β 0 -thalassemia/HbE patients who were classified as 383 severe and 235 mild phenotypes by a validated scoring system. Twenty-three SNPs in three independent genes/regions were identified as being significantly associated with the disease severity. The highest association was observed with SNPs in the β-globin gene cluster (chr.11p15), and rs2071348 of the HBBP1 gene revealed the most significant association [P = 2.96 × 10 -13 , odds ratio (OR) = 4.33 (95% confidence interval (CI), 2.74-6.84)]. The second was identified in the intergenic region between the HBS1L and MYB genes (chr.6q23), among which rs9376092 was the most significant [P = 2.36 × 10 -10 , OR = 3.07 (95% CI, 2.16-4.38)]. The third region was located in the BCL11A gene (chr.2p16.1), and rs766432 showed the most significant association [P = 5.87 × 10 -10 , OR = 3.06 (95% CI, 2.15-4.37)]. All three loci were replicated in an independent cohort of 174 Indonesian patients. The associations to fetal hemoglobin levels were also observed with SNPs on these three regions. Our data indicate that several genetic loci act in concert to influence HbF levels of β 0 -thalassemia/HbE patients. This study revealed that all the three reported loci and the α-globin gene locus are the best and common predictors of the disease severity in β-thalassemia.
| Original language | English |
|---|---|
| Pages (from-to) | 303-314 |
| Number of pages | 12 |
| Journal | Human Genetics |
| Volume | 127 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01-01-2010 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Genetics
- Genetics(clinical)
Cite this
}
A genome-wide association identified the common genetic variants influence disease severity in β 0 -thalassemia/hemoglobin e . / Nuinoon, Manit; Makarasara, Wattanan; Mushiroda, Taisei; Setianingsih, Iswari; Wahidiyat, Pustika Amalia; Sripichai, Orapan; Kumasaka, Natsuhiko; Takahashi, Atsushi; Svasti, Saovaros; Munkongdee, Thongperm; Mahasirimongkol, Surakameth; Peerapittayamongkol, Chayanon; Viprakasit, Vip; Kamatani, Naoyuki; Winichagoon, Pranee; Kubo, Michiaki; Nakamura, Yusuke; Fucharoen, Suthat.
In: Human Genetics, Vol. 127, No. 3, 01.01.2010, p. 303-314.Research output: Contribution to journal › Article
TY - JOUR
T1 - A genome-wide association identified the common genetic variants influence disease severity in β 0 -thalassemia/hemoglobin e
AU - Nuinoon, Manit
AU - Makarasara, Wattanan
AU - Mushiroda, Taisei
AU - Setianingsih, Iswari
AU - Wahidiyat, Pustika Amalia
AU - Sripichai, Orapan
AU - Kumasaka, Natsuhiko
AU - Takahashi, Atsushi
AU - Svasti, Saovaros
AU - Munkongdee, Thongperm
AU - Mahasirimongkol, Surakameth
AU - Peerapittayamongkol, Chayanon
AU - Viprakasit, Vip
AU - Kamatani, Naoyuki
AU - Winichagoon, Pranee
AU - Kubo, Michiaki
AU - Nakamura, Yusuke
AU - Fucharoen, Suthat
PY - 2010/1/1
Y1 - 2010/1/1
N2 - β-Thalassemia/HbE disease is clinically variable. In searching for genetic factors modifying the disease severity, patients were selected based on their disease severities, and a genome-wide association study (GWAS) was performed. Genotyping was conducted with the Illumina Human 610-Quad BeadChips array using DNAs from 618 Thai β 0 -thalassemia/HbE patients who were classified as 383 severe and 235 mild phenotypes by a validated scoring system. Twenty-three SNPs in three independent genes/regions were identified as being significantly associated with the disease severity. The highest association was observed with SNPs in the β-globin gene cluster (chr.11p15), and rs2071348 of the HBBP1 gene revealed the most significant association [P = 2.96 × 10 -13 , odds ratio (OR) = 4.33 (95% confidence interval (CI), 2.74-6.84)]. The second was identified in the intergenic region between the HBS1L and MYB genes (chr.6q23), among which rs9376092 was the most significant [P = 2.36 × 10 -10 , OR = 3.07 (95% CI, 2.16-4.38)]. The third region was located in the BCL11A gene (chr.2p16.1), and rs766432 showed the most significant association [P = 5.87 × 10 -10 , OR = 3.06 (95% CI, 2.15-4.37)]. All three loci were replicated in an independent cohort of 174 Indonesian patients. The associations to fetal hemoglobin levels were also observed with SNPs on these three regions. Our data indicate that several genetic loci act in concert to influence HbF levels of β 0 -thalassemia/HbE patients. This study revealed that all the three reported loci and the α-globin gene locus are the best and common predictors of the disease severity in β-thalassemia.
AB - β-Thalassemia/HbE disease is clinically variable. In searching for genetic factors modifying the disease severity, patients were selected based on their disease severities, and a genome-wide association study (GWAS) was performed. Genotyping was conducted with the Illumina Human 610-Quad BeadChips array using DNAs from 618 Thai β 0 -thalassemia/HbE patients who were classified as 383 severe and 235 mild phenotypes by a validated scoring system. Twenty-three SNPs in three independent genes/regions were identified as being significantly associated with the disease severity. The highest association was observed with SNPs in the β-globin gene cluster (chr.11p15), and rs2071348 of the HBBP1 gene revealed the most significant association [P = 2.96 × 10 -13 , odds ratio (OR) = 4.33 (95% confidence interval (CI), 2.74-6.84)]. The second was identified in the intergenic region between the HBS1L and MYB genes (chr.6q23), among which rs9376092 was the most significant [P = 2.36 × 10 -10 , OR = 3.07 (95% CI, 2.16-4.38)]. The third region was located in the BCL11A gene (chr.2p16.1), and rs766432 showed the most significant association [P = 5.87 × 10 -10 , OR = 3.06 (95% CI, 2.15-4.37)]. All three loci were replicated in an independent cohort of 174 Indonesian patients. The associations to fetal hemoglobin levels were also observed with SNPs on these three regions. Our data indicate that several genetic loci act in concert to influence HbF levels of β 0 -thalassemia/HbE patients. This study revealed that all the three reported loci and the α-globin gene locus are the best and common predictors of the disease severity in β-thalassemia.
UR - http://www.scopus.com/inward/record.url?scp=77949274495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949274495&partnerID=8YFLogxK
U2 - 10.1007/s00439-009-0770-2
DO - 10.1007/s00439-009-0770-2
M3 - Article
C2 - 20183929
AN - SCOPUS:77949274495
VL - 127
SP - 303
EP - 314
JO - Human Genetics
JF - Human Genetics
SN - 0340-6717
IS - 3
ER -