A heat shock-related protein, p20, plays an inhibitory role in platelet activation

Hiroyuki Matsuno, Osamu Kozawa, Masayuki Niwa, Akihiko Usui, Hidenori Ito, Toshihiko Uematsu, Kanefusa Kato

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30 Citations (Scopus)


Some low molecular mass heat shock proteins (HSPs) appear to act as molecular chaperones, but their exact physiological roles have not been fully elucidated. We reported previously that a 20-kDa protein (p20), which is classified as a low molecular mass HSP, is present at high levels in skeletal and smooth muscles. In the present study, we investigated a physiological role of p20 on platelet function in vitro and ex vivo. p20 inhibited platelet aggregation using human platelets dose-dependently induced by botrocetin. On the other band, HSP27, the other type of low molecular mass HSP, did not affect platelet aggregation. When p20 (300 μ/kg) was injected intravenously as a bolus in hamsters, platelet aggregation ex vivo induced by botrocetin was also significantly inhibited. In order to further investigate the inhibitory effect by p20 on platelet activation, we performed platelet aggregation induced by thrombin or ADP using human platelets. p20 markedly prevented platelet aggregation induced by thrombin, but not ADP. These findings suggest that p20 can act intercellularly to regulate platelet functions. Our results may provide the basis for a novel defensive system to thrombus formation.

Original languageEnglish
Pages (from-to)327-329
Number of pages3
JournalFEBS Letters
Issue number3
Publication statusPublished - 16-06-1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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