A herpesvirus specific motif of Epstein-Barr virus DNA polymerase is required for the efficient lytic genome synthesis

Yohei Narita, Atsuko Sugimoto, Daisuke Kawashima, Takahiro Watanabe, Teru Kanda, Hiroshi Kimura, Tatsuya Tsurumi, Takayuki Murata

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is associated with several malignancies, including Burkitt lymphoma and nasopharyngeal carcinoma. To overcome such disorders, understanding the molecular mechanisms of the EBV replication is important. The EBV DNA polymerase (Pol) is one of the essential factors for viral lytic DNA replication. Although it is well known that its C-terminal half, possessing DNA polymerase and 3′-5′ exonuclease activity, is highly conserved among Family B Pols, the NH 2 -terminal half has yet to be characterized in detail. In this study, we show that a stretch of hydrophobic amino acids within the pre-NH 2 -terminal domain of EBV Pol plays important role. In addition, we could identify the most essential residue for replication in the motif. These findings will shed light on molecular mechanisms of viral DNA synthesis and will help to develop new herpesviruses treatments.

Original languageEnglish
Article number11767
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 30-06-2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'A herpesvirus specific motif of Epstein-Barr virus DNA polymerase is required for the efficient lytic genome synthesis'. Together they form a unique fingerprint.

Cite this