A library of mutated Maackia amurensis hemagglutinin distinguishes putative glycoforms of immunoglobulin A1 from IgA nephropathy patients

Keisuke Maenuma; Mijung Yim; Kunimitsu Komatsu; Mayumi Hoshino; Ayumi Tachiki-Fujioka; Kazuo Takahashi; Yoshiyuki Hiki; Nicolai Bovin; Tatsuro Irimura

doi:10.1021/pr800816w

A library of mutated Maackia amurensis hemagglutinin distinguishes putative glycoforms of immunoglobulin A1 from IgA nephropathy patients

Keisuke Maenuma, Mijung Yim, Kunimitsu Komatsu, Mayumi Hoshino, Ayumi Tachiki-Fujioka, Kazuo Takahashi, Yoshiyuki Hiki, Nicolai Bovin, Tatsuro Irimura

Department of Biomedical Molecular Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Ten genetically modified Maackia amurensis hemagglutinin (MAH) clones at the carbohydrate-recognition loop were found to bind glycophorin A and a mucin mimetic with NeuAcα2-3Galβ1-3GalNAcα (monosialyl-T antigen) in different relative intensity. Binding profiles of these lectins to human serum IgA1 from healthy individuals and from IgA nephropathy patients were subjected to the cluster analysis. Two large groups, one with only healthy individuals and another with all IgA nephropathy patients, were generated. The results strongly suggest that the library of genetically modified MAH is a useful tool for serum diagnosis of IgA nephropathy.

Original language	English
Pages (from-to)	3617-3624
Number of pages	8
Journal	Journal of Proteome Research
Volume	8
Issue number	7
DOIs	https://doi.org/10.1021/pr800816w
Publication status	Published - 06-07-2009

All Science Journal Classification (ASJC) codes

Biochemistry
General Chemistry

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/pr800816w

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abstract = "Ten genetically modified Maackia amurensis hemagglutinin (MAH) clones at the carbohydrate-recognition loop were found to bind glycophorin A and a mucin mimetic with NeuAcα2-3Galβ1-3GalNAcα (monosialyl-T antigen) in different relative intensity. Binding profiles of these lectins to human serum IgA1 from healthy individuals and from IgA nephropathy patients were subjected to the cluster analysis. Two large groups, one with only healthy individuals and another with all IgA nephropathy patients, were generated. The results strongly suggest that the library of genetically modified MAH is a useful tool for serum diagnosis of IgA nephropathy.",

author = "Keisuke Maenuma and Mijung Yim and Kunimitsu Komatsu and Mayumi Hoshino and Ayumi Tachiki-Fujioka and Kazuo Takahashi and Yoshiyuki Hiki and Nicolai Bovin and Tatsuro Irimura",

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T1 - A library of mutated Maackia amurensis hemagglutinin distinguishes putative glycoforms of immunoglobulin A1 from IgA nephropathy patients

AU - Maenuma, Keisuke

AU - Yim, Mijung

AU - Komatsu, Kunimitsu

AU - Hoshino, Mayumi

AU - Tachiki-Fujioka, Ayumi

AU - Takahashi, Kazuo

AU - Hiki, Yoshiyuki

AU - Bovin, Nicolai

AU - Irimura, Tatsuro

PY - 2009/7/6

Y1 - 2009/7/6

N2 - Ten genetically modified Maackia amurensis hemagglutinin (MAH) clones at the carbohydrate-recognition loop were found to bind glycophorin A and a mucin mimetic with NeuAcα2-3Galβ1-3GalNAcα (monosialyl-T antigen) in different relative intensity. Binding profiles of these lectins to human serum IgA1 from healthy individuals and from IgA nephropathy patients were subjected to the cluster analysis. Two large groups, one with only healthy individuals and another with all IgA nephropathy patients, were generated. The results strongly suggest that the library of genetically modified MAH is a useful tool for serum diagnosis of IgA nephropathy.

AB - Ten genetically modified Maackia amurensis hemagglutinin (MAH) clones at the carbohydrate-recognition loop were found to bind glycophorin A and a mucin mimetic with NeuAcα2-3Galβ1-3GalNAcα (monosialyl-T antigen) in different relative intensity. Binding profiles of these lectins to human serum IgA1 from healthy individuals and from IgA nephropathy patients were subjected to the cluster analysis. Two large groups, one with only healthy individuals and another with all IgA nephropathy patients, were generated. The results strongly suggest that the library of genetically modified MAH is a useful tool for serum diagnosis of IgA nephropathy.

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A library of mutated Maackia amurensis hemagglutinin distinguishes putative glycoforms of immunoglobulin A1 from IgA nephropathy patients

Abstract

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