TY - JOUR
T1 - A liquid‐phase binding analysis for L‐selectin
T2 - A strong dependency on highly clustered sulfate groups
AU - YOSHIDA, Tomoaki
AU - FENNIE, Christopher
AU - LASKY, Laurence A.
AU - LEE, Yuan C.
PY - 1994/6
Y1 - 1994/6
N2 - L‐selectin, together with E‐ and P‐selectins, forms a newer group of cell adhesion molecules which are believed to interact with carbohydrate ligands [Lasky, L. A., Singer, M. S., Yednoch, T. A., Dowbenko, D., Fennie, C., Rodriguez, H., Nguyen, T., Stachel, S. & Rosen, S. D. (1989) Cell 56, 1045–1055]. Using radiolabeled fucoidan as a reference ligand, we have developed a new liquid‐phase microcentrifugation assay where fine differences in binding affinity can be compared accurately. We found that glucan sulfates strongly inhibited the binding of fucoidan by murine L‐selectin‐IgG chimera. The efficacy of inhibition is extremely dependent on the size (up to 12 kDa) and the sulfate density (up to two sulfate groups/glucose molecule) of the glucan sulfates. The nature of the inter‐glucose linkages is also important. These data suggest that the binding by L‐selectin prefers certain clustering and proper spatial arrangement of the anionic groups.
AB - L‐selectin, together with E‐ and P‐selectins, forms a newer group of cell adhesion molecules which are believed to interact with carbohydrate ligands [Lasky, L. A., Singer, M. S., Yednoch, T. A., Dowbenko, D., Fennie, C., Rodriguez, H., Nguyen, T., Stachel, S. & Rosen, S. D. (1989) Cell 56, 1045–1055]. Using radiolabeled fucoidan as a reference ligand, we have developed a new liquid‐phase microcentrifugation assay where fine differences in binding affinity can be compared accurately. We found that glucan sulfates strongly inhibited the binding of fucoidan by murine L‐selectin‐IgG chimera. The efficacy of inhibition is extremely dependent on the size (up to 12 kDa) and the sulfate density (up to two sulfate groups/glucose molecule) of the glucan sulfates. The nature of the inter‐glucose linkages is also important. These data suggest that the binding by L‐selectin prefers certain clustering and proper spatial arrangement of the anionic groups.
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U2 - 10.1111/j.1432-1033.1994.tb18915.x
DO - 10.1111/j.1432-1033.1994.tb18915.x
M3 - Article
C2 - 7517358
AN - SCOPUS:0028235341
SN - 0014-2956
VL - 222
SP - 703
EP - 709
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -