A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes

Ken Ohashi, Fumio Takizawa, Norihiro Tokumaru, Chihaya Nakayasu, Hideaki Toda, Uwe Fischer, Tadaaki Moritomo, Keiichiro Hashimoto, Teruyuki Nakanishi, J・m Dijkstra

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In teleost fish, a novel gene G6F-like was identified, encoding a type I transmembrane molecule with four extracellular Ig-like domains and a cytoplasmic tail with putative tyrosine phosphorylation motifs including YxN and an immunoreceptor tyrosine-based activation motif (ITAM). G6F-like maps to a teleost genomic region where stretches corresponding to human chromosomes 6p (with the MHC), 12p (with CD4 and LAG-3), and 19q are tightly linked. This genomic organization resembles the ancestral "Ur-MHC" proposed for the jawed vertebrate ancestor. The deduced G6F-like molecule shows sequence similarity with members of the CD4/LAG-3 family and with the human major histocompatibility complex-encoded thrombocyte marker G6F. Despite some differences in molecular organization, teleost G6F-like and tetrapod G6F seem orthologous as they map to similar genomic location, share typical motifs in transmembrane and cytoplasmic regions, and are both expressed by thrombocytes/platelets. In the crucian carps goldfish (Carassius auratus auratus) and ginbuna (Carassius auratus langsdorfii), G6F-like was found expressed not only by thrombocytes but also by erythrocytes, supporting that erythroid and thromboid cells in teleost fish form a hematopoietic lineage like they do in mammals. The ITAM-bearing of G6F-like suggests that the molecule plays an important role in cell activation, and G6F-like expression by erythrocytes suggests that these cells have functional overlap potential with thrombocytes.

Original languageEnglish
Pages (from-to)543-559
Number of pages17
JournalImmunogenetics
Volume62
Issue number8
DOIs
Publication statusPublished - 01-08-2010

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Immunoreceptor Tyrosine-Based Activation Motif
Tail
Fishes
Blood Platelets
Erythrocytes
Goldfish
Erythroid Cells
Carps
Human Chromosomes
Major Histocompatibility Complex
Tyrosine
Vertebrates
Mammals
Phosphorylation
Genes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Ohashi, Ken ; Takizawa, Fumio ; Tokumaru, Norihiro ; Nakayasu, Chihaya ; Toda, Hideaki ; Fischer, Uwe ; Moritomo, Tadaaki ; Hashimoto, Keiichiro ; Nakanishi, Teruyuki ; Dijkstra, J・m. / A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes. In: Immunogenetics. 2010 ; Vol. 62, No. 8. pp. 543-559.
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abstract = "In teleost fish, a novel gene G6F-like was identified, encoding a type I transmembrane molecule with four extracellular Ig-like domains and a cytoplasmic tail with putative tyrosine phosphorylation motifs including YxN and an immunoreceptor tyrosine-based activation motif (ITAM). G6F-like maps to a teleost genomic region where stretches corresponding to human chromosomes 6p (with the MHC), 12p (with CD4 and LAG-3), and 19q are tightly linked. This genomic organization resembles the ancestral {"}Ur-MHC{"} proposed for the jawed vertebrate ancestor. The deduced G6F-like molecule shows sequence similarity with members of the CD4/LAG-3 family and with the human major histocompatibility complex-encoded thrombocyte marker G6F. Despite some differences in molecular organization, teleost G6F-like and tetrapod G6F seem orthologous as they map to similar genomic location, share typical motifs in transmembrane and cytoplasmic regions, and are both expressed by thrombocytes/platelets. In the crucian carps goldfish (Carassius auratus auratus) and ginbuna (Carassius auratus langsdorfii), G6F-like was found expressed not only by thrombocytes but also by erythrocytes, supporting that erythroid and thromboid cells in teleost fish form a hematopoietic lineage like they do in mammals. The ITAM-bearing of G6F-like suggests that the molecule plays an important role in cell activation, and G6F-like expression by erythrocytes suggests that these cells have functional overlap potential with thrombocytes.",
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A molecule in teleost fish, related with human MHC-encoded G6F, has a cytoplasmic tail with ITAM and marks the surface of thrombocytes and in some fishes also of erythrocytes. / Ohashi, Ken; Takizawa, Fumio; Tokumaru, Norihiro; Nakayasu, Chihaya; Toda, Hideaki; Fischer, Uwe; Moritomo, Tadaaki; Hashimoto, Keiichiro; Nakanishi, Teruyuki; Dijkstra, J・m.

In: Immunogenetics, Vol. 62, No. 8, 01.08.2010, p. 543-559.

Research output: Contribution to journalArticle

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