A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy

Yasuhiko Taki, Atsushi Fuku, Yuka Nakamura, Terutsugu Koya, Hironori Kitajima, Ikuhiro Tanida, Takashi Takaki, Kaori Nozaki, Hiroshi Sunami, Hiroaki Hirata, Yoshiyuki Tachi, Takeo Shimasaki, Togen Masauji, Naoki Yamamoto, Yasuhito Ishigaki, Shigetaka Shimodaira, Yusuke Shimizu, Toru Ichiseki, Ayumi Kaneuji, Satoshi OsawaNorio Kawahara

Research output: Contribution to journalArticlepeer-review

Abstract

Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet composed of human ADSCs could be created using a new temperature-responsive culture dish from the DIC Corporation. The dish can cause detachment of adherent cells due to temperature changes, but a few morphological analyses have evaluated the presence or absence of damage on the detached surface of cell sheet. To characterize our ADSC sheet, we tried to observe the surface of ADSC sheets with scanning electron microscope (SEM) using the ionic liquid, which enables the rapid preparation of samples. No damage was found on the surface of the ADSC sheets on the side that had been in contact with the surface of the culture dishes. In addition, when the transcriptomes of the harvested cell sheets were compared with those of monolayer cultures, no up-regulation of cell death related genes were detected. These results propose that the detachment from temperature-responsive culture dish causes no serious damage on the prepared ADSC sheet. It is also suggested that the SEM with ionic liquids is a useful and rapid method for the analysis of ADSC sheets for therapy.

Original languageEnglish
Pages (from-to)187-198
Number of pages12
JournalMedical Molecular Morphology
Volume55
Issue number3
DOIs
Publication statusPublished - 09-2022

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology

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