A new gerbil model of hindbrain ischemia by extracranial occlusion of the bilateral vertebral arteries

Ryuji Hata, Masayasu Matsumoto, Kazuo Kitagawa, Tomohiro Matsuyama, Toshiho Ohtsuki, Masafumi Tagaya, Nobuo Handa, Michio Niinobe, Katsuhiko Mikoshiba, Tsunehiko Nishimura, Takehiko Yanagihara, Takenobu Kamada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A new gerbil model of hindbrain ischemia was induced by extracranial occlusion of the bilateral vertebral arteries just before their entry into the transverse foramen of the cervical vertebra. Carbon black studies, performed at 5 min after occlusion, revealed that the pons-medulla oblongata, and the cerebellum were quite ischemic in all animals. Cardiovascular changes in mean arterial blood pressure (MABP) and heart rate were recorded until 30 min after occlusion, and revealed that the typical cerebral ischemic response (i.e., abrupt increase in MABP, bradycardia, and apnea) was elicited in all animals (n = 10). Thirty minutes after occlusion, animals (n = 4) were decapitated and immersion-fixed. Brain sections were stained with hematoxylin-eosin (HE) and also immunostained for microtubule-associated protein 2 in order to evaluate ischemic neuronal damage from 30 min of ischemia. By HE staining, ischemic lesions were detected bilaterally in the oculomotor, the trigeminal motor, the lateral vestibular, and the cerebellar interpositus nucleus. In addition, immunostaining revealed ischemic lesions in several other hindbrain areas. In conclusion, we could successfully establish a new gerbil model of hindbrain ischemia. Carbon black perfusion and hemodynamic studies revealed that severe and reproducible hindbrain ischemia was produced. By histopathological examination, we could also clearly demonstrate symmetrical ischemic lesions in several hindbrain areas.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalJournal of the Neurological Sciences
Volume121
Issue number1
DOIs
Publication statusPublished - 01-01-1994

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Rhombencephalon
Vertebral Artery
Gerbillinae
Ischemia
Arterial Pressure
Soot
Hematoxylin
Eosine Yellowish-(YS)
Cervical Vertebrae
Cerebellar Nuclei
Medulla Oblongata
Microtubule-Associated Proteins
Pons
Immersion
Apnea
Bradycardia
Cerebellum
Perfusion
Heart Rate
Hemodynamics

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Hata, Ryuji ; Matsumoto, Masayasu ; Kitagawa, Kazuo ; Matsuyama, Tomohiro ; Ohtsuki, Toshiho ; Tagaya, Masafumi ; Handa, Nobuo ; Niinobe, Michio ; Mikoshiba, Katsuhiko ; Nishimura, Tsunehiko ; Yanagihara, Takehiko ; Kamada, Takenobu. / A new gerbil model of hindbrain ischemia by extracranial occlusion of the bilateral vertebral arteries. In: Journal of the Neurological Sciences. 1994 ; Vol. 121, No. 1. pp. 79-89.
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abstract = "A new gerbil model of hindbrain ischemia was induced by extracranial occlusion of the bilateral vertebral arteries just before their entry into the transverse foramen of the cervical vertebra. Carbon black studies, performed at 5 min after occlusion, revealed that the pons-medulla oblongata, and the cerebellum were quite ischemic in all animals. Cardiovascular changes in mean arterial blood pressure (MABP) and heart rate were recorded until 30 min after occlusion, and revealed that the typical cerebral ischemic response (i.e., abrupt increase in MABP, bradycardia, and apnea) was elicited in all animals (n = 10). Thirty minutes after occlusion, animals (n = 4) were decapitated and immersion-fixed. Brain sections were stained with hematoxylin-eosin (HE) and also immunostained for microtubule-associated protein 2 in order to evaluate ischemic neuronal damage from 30 min of ischemia. By HE staining, ischemic lesions were detected bilaterally in the oculomotor, the trigeminal motor, the lateral vestibular, and the cerebellar interpositus nucleus. In addition, immunostaining revealed ischemic lesions in several other hindbrain areas. In conclusion, we could successfully establish a new gerbil model of hindbrain ischemia. Carbon black perfusion and hemodynamic studies revealed that severe and reproducible hindbrain ischemia was produced. By histopathological examination, we could also clearly demonstrate symmetrical ischemic lesions in several hindbrain areas.",
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Hata, R, Matsumoto, M, Kitagawa, K, Matsuyama, T, Ohtsuki, T, Tagaya, M, Handa, N, Niinobe, M, Mikoshiba, K, Nishimura, T, Yanagihara, T & Kamada, T 1994, 'A new gerbil model of hindbrain ischemia by extracranial occlusion of the bilateral vertebral arteries', Journal of the Neurological Sciences, vol. 121, no. 1, pp. 79-89. https://doi.org/10.1016/0022-510X(94)90160-0

A new gerbil model of hindbrain ischemia by extracranial occlusion of the bilateral vertebral arteries. / Hata, Ryuji; Matsumoto, Masayasu; Kitagawa, Kazuo; Matsuyama, Tomohiro; Ohtsuki, Toshiho; Tagaya, Masafumi; Handa, Nobuo; Niinobe, Michio; Mikoshiba, Katsuhiko; Nishimura, Tsunehiko; Yanagihara, Takehiko; Kamada, Takenobu.

In: Journal of the Neurological Sciences, Vol. 121, No. 1, 01.01.1994, p. 79-89.

Research output: Contribution to journalArticle

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T1 - A new gerbil model of hindbrain ischemia by extracranial occlusion of the bilateral vertebral arteries

AU - Hata, Ryuji

AU - Matsumoto, Masayasu

AU - Kitagawa, Kazuo

AU - Matsuyama, Tomohiro

AU - Ohtsuki, Toshiho

AU - Tagaya, Masafumi

AU - Handa, Nobuo

AU - Niinobe, Michio

AU - Mikoshiba, Katsuhiko

AU - Nishimura, Tsunehiko

AU - Yanagihara, Takehiko

AU - Kamada, Takenobu

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