A new reliable method for detecting specific IgE antibodies in the patients with immediate type wheat allergy due to hydrolyzed wheat protein: Correlation of its titer and clinical severity

Masashi Nakamura, Akiko Yagami, Kazuhiro Hara, Akiyo Sano, Tsukane Kobayashi, Michiko Aihara, Michihiro Hide, Yuko Chinuki, Eishin Morita, Reiko Teshima, Kayoko Matsunaga

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Immediate-type wheat allergy caused by a specific hydrolyzed wheat protein (HWP-IWA), Glupearl 19S (GP19S), typically develops food-dependent exercise-induced anaphylaxis (FDEIA), but is different from conventional FDEIA, or simple wheat allergy in many aspects. The skin prick test (SPT) is considered to be the most effective method for diagnosis of HWP-IWA. As SPT is a relatively qualitative method, we developed quantitative and high-throughput test method for HWP-IWA. Methods: An enzyme-linked immunosorbent assay (ELISA)-based GP19S-specific IgE assay was tested using sera from 14 HWP-IWA and five conventional wheat-dependent exercise-induced anaphylaxis (CO-WDEIA) patients, as well as five healthy subjects. Then a validation study at five different institutions was carried out using sera from 10 HWP-IWA and five CO-WDEIA patients, as well as five healthy subjects different from the previous studies. Results: The mean unit values converted from measured absorbance of ELISA were 68.3, 1.3 and 1.1 respectively. Furthermore, the validation study revealed reproducible results across all five institutions, with the standard deviation (SD) being 0.3-0.4 for the healthy group, 0.2-0.6 for the CO-WDEIA group, and 3.8-9.6 for HWP-IWA group except for one case. One case of HWP-IWA was excluded from analysis due to the high SD of 53.3 units, indicating that samples with a unit value > 100.0 will affect inter-laboratory reproducibility. Conclusions: Our findings suggest that the ELISA-based GP19S-specific IgE assay can be used to test HWP-IWA using venous blood samples, except for those with a unit value > 100.0.

Original languageEnglish
Pages (from-to)243-249
Number of pages7
JournalAllergology International
Volume63
Issue number2
DOIs
Publication statusPublished - 01-01-2014

Fingerprint

Wheat Hypersensitivity
Anaphylaxis
Immunoglobulin E
Triticum
Exercise
Antibodies
Validation Studies
Enzyme-Linked Immunosorbent Assay
Skin Tests
Proteins
Healthy Volunteers
Food
Serum
Glupearl 19S

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy

Cite this

Nakamura, Masashi ; Yagami, Akiko ; Hara, Kazuhiro ; Sano, Akiyo ; Kobayashi, Tsukane ; Aihara, Michiko ; Hide, Michihiro ; Chinuki, Yuko ; Morita, Eishin ; Teshima, Reiko ; Matsunaga, Kayoko. / A new reliable method for detecting specific IgE antibodies in the patients with immediate type wheat allergy due to hydrolyzed wheat protein : Correlation of its titer and clinical severity. In: Allergology International. 2014 ; Vol. 63, No. 2. pp. 243-249.
@article{1eba00ffa17848c2aa7ecf454e1b396b,
title = "A new reliable method for detecting specific IgE antibodies in the patients with immediate type wheat allergy due to hydrolyzed wheat protein: Correlation of its titer and clinical severity",
abstract = "Background: Immediate-type wheat allergy caused by a specific hydrolyzed wheat protein (HWP-IWA), Glupearl 19S (GP19S), typically develops food-dependent exercise-induced anaphylaxis (FDEIA), but is different from conventional FDEIA, or simple wheat allergy in many aspects. The skin prick test (SPT) is considered to be the most effective method for diagnosis of HWP-IWA. As SPT is a relatively qualitative method, we developed quantitative and high-throughput test method for HWP-IWA. Methods: An enzyme-linked immunosorbent assay (ELISA)-based GP19S-specific IgE assay was tested using sera from 14 HWP-IWA and five conventional wheat-dependent exercise-induced anaphylaxis (CO-WDEIA) patients, as well as five healthy subjects. Then a validation study at five different institutions was carried out using sera from 10 HWP-IWA and five CO-WDEIA patients, as well as five healthy subjects different from the previous studies. Results: The mean unit values converted from measured absorbance of ELISA were 68.3, 1.3 and 1.1 respectively. Furthermore, the validation study revealed reproducible results across all five institutions, with the standard deviation (SD) being 0.3-0.4 for the healthy group, 0.2-0.6 for the CO-WDEIA group, and 3.8-9.6 for HWP-IWA group except for one case. One case of HWP-IWA was excluded from analysis due to the high SD of 53.3 units, indicating that samples with a unit value > 100.0 will affect inter-laboratory reproducibility. Conclusions: Our findings suggest that the ELISA-based GP19S-specific IgE assay can be used to test HWP-IWA using venous blood samples, except for those with a unit value > 100.0.",
author = "Masashi Nakamura and Akiko Yagami and Kazuhiro Hara and Akiyo Sano and Tsukane Kobayashi and Michiko Aihara and Michihiro Hide and Yuko Chinuki and Eishin Morita and Reiko Teshima and Kayoko Matsunaga",
year = "2014",
month = "1",
day = "1",
doi = "10.2332/allergolint.13-OA-0618",
language = "English",
volume = "63",
pages = "243--249",
journal = "Allergology International",
issn = "1323-8930",
publisher = "Japanese Society of Allergology",
number = "2",

}

A new reliable method for detecting specific IgE antibodies in the patients with immediate type wheat allergy due to hydrolyzed wheat protein : Correlation of its titer and clinical severity. / Nakamura, Masashi; Yagami, Akiko; Hara, Kazuhiro; Sano, Akiyo; Kobayashi, Tsukane; Aihara, Michiko; Hide, Michihiro; Chinuki, Yuko; Morita, Eishin; Teshima, Reiko; Matsunaga, Kayoko.

In: Allergology International, Vol. 63, No. 2, 01.01.2014, p. 243-249.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A new reliable method for detecting specific IgE antibodies in the patients with immediate type wheat allergy due to hydrolyzed wheat protein

T2 - Correlation of its titer and clinical severity

AU - Nakamura, Masashi

AU - Yagami, Akiko

AU - Hara, Kazuhiro

AU - Sano, Akiyo

AU - Kobayashi, Tsukane

AU - Aihara, Michiko

AU - Hide, Michihiro

AU - Chinuki, Yuko

AU - Morita, Eishin

AU - Teshima, Reiko

AU - Matsunaga, Kayoko

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Immediate-type wheat allergy caused by a specific hydrolyzed wheat protein (HWP-IWA), Glupearl 19S (GP19S), typically develops food-dependent exercise-induced anaphylaxis (FDEIA), but is different from conventional FDEIA, or simple wheat allergy in many aspects. The skin prick test (SPT) is considered to be the most effective method for diagnosis of HWP-IWA. As SPT is a relatively qualitative method, we developed quantitative and high-throughput test method for HWP-IWA. Methods: An enzyme-linked immunosorbent assay (ELISA)-based GP19S-specific IgE assay was tested using sera from 14 HWP-IWA and five conventional wheat-dependent exercise-induced anaphylaxis (CO-WDEIA) patients, as well as five healthy subjects. Then a validation study at five different institutions was carried out using sera from 10 HWP-IWA and five CO-WDEIA patients, as well as five healthy subjects different from the previous studies. Results: The mean unit values converted from measured absorbance of ELISA were 68.3, 1.3 and 1.1 respectively. Furthermore, the validation study revealed reproducible results across all five institutions, with the standard deviation (SD) being 0.3-0.4 for the healthy group, 0.2-0.6 for the CO-WDEIA group, and 3.8-9.6 for HWP-IWA group except for one case. One case of HWP-IWA was excluded from analysis due to the high SD of 53.3 units, indicating that samples with a unit value > 100.0 will affect inter-laboratory reproducibility. Conclusions: Our findings suggest that the ELISA-based GP19S-specific IgE assay can be used to test HWP-IWA using venous blood samples, except for those with a unit value > 100.0.

AB - Background: Immediate-type wheat allergy caused by a specific hydrolyzed wheat protein (HWP-IWA), Glupearl 19S (GP19S), typically develops food-dependent exercise-induced anaphylaxis (FDEIA), but is different from conventional FDEIA, or simple wheat allergy in many aspects. The skin prick test (SPT) is considered to be the most effective method for diagnosis of HWP-IWA. As SPT is a relatively qualitative method, we developed quantitative and high-throughput test method for HWP-IWA. Methods: An enzyme-linked immunosorbent assay (ELISA)-based GP19S-specific IgE assay was tested using sera from 14 HWP-IWA and five conventional wheat-dependent exercise-induced anaphylaxis (CO-WDEIA) patients, as well as five healthy subjects. Then a validation study at five different institutions was carried out using sera from 10 HWP-IWA and five CO-WDEIA patients, as well as five healthy subjects different from the previous studies. Results: The mean unit values converted from measured absorbance of ELISA were 68.3, 1.3 and 1.1 respectively. Furthermore, the validation study revealed reproducible results across all five institutions, with the standard deviation (SD) being 0.3-0.4 for the healthy group, 0.2-0.6 for the CO-WDEIA group, and 3.8-9.6 for HWP-IWA group except for one case. One case of HWP-IWA was excluded from analysis due to the high SD of 53.3 units, indicating that samples with a unit value > 100.0 will affect inter-laboratory reproducibility. Conclusions: Our findings suggest that the ELISA-based GP19S-specific IgE assay can be used to test HWP-IWA using venous blood samples, except for those with a unit value > 100.0.

UR - http://www.scopus.com/inward/record.url?scp=84901987562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901987562&partnerID=8YFLogxK

U2 - 10.2332/allergolint.13-OA-0618

DO - 10.2332/allergolint.13-OA-0618

M3 - Article

C2 - 24759554

AN - SCOPUS:84901987562

VL - 63

SP - 243

EP - 249

JO - Allergology International

JF - Allergology International

SN - 1323-8930

IS - 2

ER -