A new retrovirus produced by tissue culture cell line from mammary tumor of a house musk shrew, Suncus murinus

Yoshihiro Tsutsui, Shoichi Nagayoshi, Shinsuke Saga, Masahide Takahashi, Atsuko Aoyama, Jollyanna Yamashiro Malavasi, Seijun Hayakawa, Toyoharu Yokoi, Sen Ichi Oda, Munemitsu Hoshino

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Abstract

A new type of retrovirus (Sm-MTV) released by cultured cells of a spontaneous mammary tumor from a house musk shrew, Suncus murinus, is described. The Sm-MTV is distinct morphologically from type C particles. In spite of certain morphological similarities to type B and type D retroviruses, the Sm-MTV is readily distinguishable. The extracellular virions had a spikeless envelope containing a centrally located nucleoid with a small electron-dense core surrounded by an inner membrane. The budding particles contained a doughnut-shaped nucleoid. Intracytoplasmic type A particles similar in profile to those associated with mouse mammary tumor cells were also found, and tended to form a small cluster of several particles in the cytoplasm. The virus banded at 1.169 g/cm3 in isopycnic centrifugation and possessed constitutive Mg2+-dependent reverse transcriptase. The viral RNA had a molecular size ranging from 50 to 70 S in its native form and 30 to 40 S in its denatured form by a glycerol gradient ultracentrifugation. Major viral polypeptides were 72K, 69K, 47K, 44K/43K, 27K, 20.5K, and 15K.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalVirology
Volume144
Issue number1
DOIs
Publication statusPublished - 15-07-1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

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    Tsutsui, Y., Nagayoshi, S., Saga, S., Takahashi, M., Aoyama, A., Malavasi, J. Y., Hayakawa, S., Yokoi, T., Oda, S. I., & Hoshino, M. (1985). A new retrovirus produced by tissue culture cell line from mammary tumor of a house musk shrew, Suncus murinus. Virology, 144(1), 273-278. https://doi.org/10.1016/0042-6822(85)90324-1