A new system for the expression of recombinant antibody in mammalian cells

Yoshitaka Iba, Takashi Kaneko, Teiji Ekida, Kenji Miyata, Kuniyo Inouye, Yoshikazu Kurosawa, Kiyoshi Yasukawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

Original languageEnglish
Pages (from-to)135-138
Number of pages4
JournalBiotechnology Letters
Volume17
Issue number2
DOIs
Publication statusPublished - 01-02-1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'A new system for the expression of recombinant antibody in mammalian cells'. Together they form a unique fingerprint.

Cite this