Abstract
New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.
Original language | English |
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Pages (from-to) | 135-138 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 17 |
Issue number | 2 |
DOIs | |
Publication status | Published - 02-1995 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology