A new system for the expression of recombinant antibody in mammalian cells

Yoshitaka Iba; Takashi Kaneko; Teiji Ekida; Kenji Miyata; Kuniyo Inouye; Yoshikazu Kurosawa; Kiyoshi Yasukawa

doi:10.1007/BF00127976

A new system for the expression of recombinant antibody in mammalian cells

Yoshitaka Iba
, Takashi Kaneko
, Teiji Ekida
, Kenji Miyata
, Kuniyo Inouye
, Yoshikazu Kurosawa
, Kiyoshi Yasukawa

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

Original language	English
Pages (from-to)	135-138
Number of pages	4
Journal	Biotechnology Letters
Volume	17
Issue number	2
DOIs	https://doi.org/10.1007/BF00127976
Publication status	Published - 02-1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/BF00127976

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title = "A new system for the expression of recombinant antibody in mammalian cells",

abstract = "New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.",

author = "Yoshitaka Iba and Takashi Kaneko and Teiji Ekida and Kenji Miyata and Kuniyo Inouye and Yoshikazu Kurosawa and Kiyoshi Yasukawa",

year = "1995",

month = feb,

doi = "10.1007/BF00127976",

language = "English",

volume = "17",

pages = "135--138",

journal = "Biotechnology Letters",

issn = "0141-5492",

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TY - JOUR

T1 - A new system for the expression of recombinant antibody in mammalian cells

AU - Iba, Yoshitaka

AU - Kaneko, Takashi

AU - Ekida, Teiji

AU - Miyata, Kenji

AU - Inouye, Kuniyo

AU - Kurosawa, Yoshikazu

AU - Yasukawa, Kiyoshi

PY - 1995/2

Y1 - 1995/2

N2 - New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

AB - New type of plasmids named pEdHCG1 and pEdHCκ.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

UR - https://www.scopus.com/pages/publications/0028951087

UR - https://www.scopus.com/pages/publications/0028951087#tab=citedBy

U2 - 10.1007/BF00127976

DO - 10.1007/BF00127976

M3 - Article

AN - SCOPUS:0028951087

SN - 0141-5492

VL - 17

SP - 135

EP - 138

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 2

ER -

A new system for the expression of recombinant antibody in mammalian cells

Abstract

All Science Journal Classification (ASJC) codes

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