A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance

Hiroshi Kurokawa, Naohiro Shibata, Yohei Doi, Keigo Shibayama, Kazunari Kamachi, Tetsuya Yagi, Yoshichika Arakawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.

Original languageEnglish
Pages (from-to)2981-2983
Number of pages3
JournalAntimicrobial agents and chemotherapy
Volume47
Issue number9
DOIs
Publication statusPublished - 01-09-2003

Fingerprint

Ceftazidime
Penicillinase
Clavulanic Acid
Isoelectric Point
Amino Acid Substitution
Japan
Hydrolysis
Plasmids
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Kurokawa, Hiroshi ; Shibata, Naohiro ; Doi, Yohei ; Shibayama, Keigo ; Kamachi, Kazunari ; Yagi, Tetsuya ; Arakawa, Yoshichika. / A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance. In: Antimicrobial agents and chemotherapy. 2003 ; Vol. 47, No. 9. pp. 2981-2983.
@article{c799b0b0c337467aad604b3fffa2b310,
title = "A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance",
abstract = "A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.",
author = "Hiroshi Kurokawa and Naohiro Shibata and Yohei Doi and Keigo Shibayama and Kazunari Kamachi and Tetsuya Yagi and Yoshichika Arakawa",
year = "2003",
month = "9",
day = "1",
doi = "10.1128/AAC.47.9.2981-2983.2003",
language = "English",
volume = "47",
pages = "2981--2983",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "9",

}

A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance. / Kurokawa, Hiroshi; Shibata, Naohiro; Doi, Yohei; Shibayama, Keigo; Kamachi, Kazunari; Yagi, Tetsuya; Arakawa, Yoshichika.

In: Antimicrobial agents and chemotherapy, Vol. 47, No. 9, 01.09.2003, p. 2981-2983.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance

AU - Kurokawa, Hiroshi

AU - Shibata, Naohiro

AU - Doi, Yohei

AU - Shibayama, Keigo

AU - Kamachi, Kazunari

AU - Yagi, Tetsuya

AU - Arakawa, Yoshichika

PY - 2003/9/1

Y1 - 2003/9/1

N2 - A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.

AB - A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.

UR - http://www.scopus.com/inward/record.url?scp=0041922557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041922557&partnerID=8YFLogxK

U2 - 10.1128/AAC.47.9.2981-2983.2003

DO - 10.1128/AAC.47.9.2981-2983.2003

M3 - Article

C2 - 12937007

AN - SCOPUS:0041922557

VL - 47

SP - 2981

EP - 2983

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 9

ER -