TY - JOUR
T1 - A new TEM-derived extended-spectrum β-lactamase (TEM-91) with an R164C substitution at the ω-loop confers ceftazidime resistance
AU - Kurokawa, Hiroshi
AU - Shibata, Naohiro
AU - Doi, Yohei
AU - Shibayama, Keigo
AU - Kamachi, Kazunari
AU - Yagi, Tetsuya
AU - Arakawa, Yoshichika
PY - 2003/9/1
Y1 - 2003/9/1
N2 - A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.
AB - A new plasmid-mediated TEM-derived extended-spectrum β-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 μg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), Km, and kcat of TEM-91 for ceftazidime were 5.7, 179 μM, and 29.0 s-1, respectively. The Ki of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.
UR - http://www.scopus.com/inward/record.url?scp=0041922557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041922557&partnerID=8YFLogxK
U2 - 10.1128/AAC.47.9.2981-2983.2003
DO - 10.1128/AAC.47.9.2981-2983.2003
M3 - Article
C2 - 12937007
AN - SCOPUS:0041922557
SN - 0066-4804
VL - 47
SP - 2981
EP - 2983
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 9
ER -