Abstract
Human chymase produces a novel endothelin-1 with 31 amino-acid length {ET-1(1-31)}, which is longer than conventional ET-1, {ET-1(1-21)}. The aim of our study was to investigate the role of ET-1(1-31) on porcine coronary vascular smooth muscle cell (VSMC). Although the increase in [Ca2+](i) by ET-1(1-31) was 10 times weaker than that of ET-1(1-21), ET-1(1-31) showed equivalent potency in VSMC proliferation, c-fos/c-myc mRNA expression and cell cycle analysis with ET-1(1-21). ET-1(1-31) significantly induced expression of cyclin D1 but not those of cyclin D2 or D3. These effects were specifically inhibited by BQ485, an ET(A) receptor antagonist, although that of ET-1(1-21) was not specific to BQ485, suggesting different receptor specificity from ET-1(1-21). These results indicate that ET-1(1-31) also can involve a VSMC proliferation process such as atherosclerosis, although it has weaker vasoconstricting potency and different receptor subtypes on VSMC from those of ET-1(1-21). (C) 2000 Academic Press.
Original language | English |
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Pages (from-to) | 595-600 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 275 |
Issue number | 2 |
DOIs | |
Publication status | Published - 28-08-2000 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology