A novel 31-amino-acid-length endothelin, ET-1(1-31), can act as a biologically active peptide for vascular smooth muscle cells

Noriko Nagata, Yasuharu Niwa, Yutaka Nakaya

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Human chymase produces a novel endothelin-1 with 31 amino-acid length {ET-1(1-31)}, which is longer than conventional ET-1, {ET-1(1-21)}. The aim of our study was to investigate the role of ET-1(1-31) on porcine coronary vascular smooth muscle cell (VSMC). Although the increase in [Ca2+](i) by ET-1(1-31) was 10 times weaker than that of ET-1(1-21), ET-1(1-31) showed equivalent potency in VSMC proliferation, c-fos/c-myc mRNA expression and cell cycle analysis with ET-1(1-21). ET-1(1-31) significantly induced expression of cyclin D1 but not those of cyclin D2 or D3. These effects were specifically inhibited by BQ485, an ET(A) receptor antagonist, although that of ET-1(1-21) was not specific to BQ485, suggesting different receptor specificity from ET-1(1-21). These results indicate that ET-1(1-31) also can involve a VSMC proliferation process such as atherosclerosis, although it has weaker vasoconstricting potency and different receptor subtypes on VSMC from those of ET-1(1-21). (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)595-600
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number2
DOIs
Publication statusPublished - 28-08-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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