A novel caspase dependent pathway is involved in apoptosis of human endothelial cells by Shiga toxins

Tomoaki Yoshida, Naoki Koide, Tsuyoshi Sugiyama, Isamu Mori, Takashi Yokochi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Shiga toxins have been shown to induce apoptosis on primary cultures, but not passaged ones, of human umbilical vein endothelial cells, independent of cytokine pre-treatment. Here, a peculiar pattern of caspase activation was observed; caspase-3 and -2, but not conventional upstream caspases, were activated at the initial phase of 6 hr, whereas a broad range inhibitor of caspases, VAD-fmk, but not mono-specific ones, suppressed DNA fragmentation and cell death. These results suggest additional analogous molecules, which have yet to be delineated, are involved. The requirement of retrograde uptake of toxins was also proved by the intervening effect of brefeldin A.

Original languageEnglish
Pages (from-to)697-700
Number of pages4
JournalMICROBIOLOGY and IMMUNOLOGY
Volume46
Issue number10
DOIs
Publication statusPublished - 01-01-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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