A novel combination RNAi toward warburg effect by replacement with miR-145 and silencing of PTBP1 induces apoptotic cell death in bladder cancer cells

Tomoaki Takai, Yuki Yoshikawa, Teruo Inamoto, Koichiro Minami, Kohei Taniguchi, Nobuhiko Sugito, Yuki Kuranaga, Haruka Shinohara, Minami Kumazaki, Takuya Tsujino, Kiyoshi Takahara, Yuko Ito, Yukihiro Akao, Haruhito Azuma

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Bladder cancer is one of the most difficult malignancies to control. We explored the use of a novel RNA-interference method for a driver oncogene regulating cancer specific energy metabolism by the combination treatment with a small interfering RNA (siRNA) and a microRNA. After transfection of T24 and 253JB-V cells with miR-145 and/or siR-PTBP1, we examined the effects of cell growth and gene expression by performing the trypan blue dye exclusion test, Western blot, Hoechst 33342 staining, reverse transcription polymerase chain reaction (RT-PCR), and electron microscopy. The anti-cancer effects of xenograft model mice with miR-145 and/or siR-PTBP1 were then assessed. The combination treatment induced the deeper and longer growth inhibition and reduced the levels of both mRNA and protein expression of c-Myc and polypyrimidine tract-binding protein 1 (PTBP1) more than each single treatment. Notably, the combination treatment not only impaired the cancer specific energy metabolism by inhibiting c-Myc/PTBP1/PKMs axis but also inactivated MAPK/ERK and PI3K/AKT pathways examined in vitro and in vivo. Furthermore, the combination treatment induced apoptosis or autophagy; but, in some cells, apoptotic cell death was accompanied by autophagy, because the condensation of chromatin and many autophagosomes were coexistent. This combination treatment could be a novel RNA-interference strategy through the systemic silencing of the Warburg effect-promoting driver oncogene PTBP1 in bladder cancer cells.

Original languageEnglish
Article number179
JournalInternational Journal of Molecular Sciences
Volume18
Issue number1
DOIs
Publication statusPublished - 17-01-2017
Externally publishedYes

Fingerprint

Polypyrimidine Tract-Binding Protein
bladder
Cell death
RNA Interference
Urinary Bladder Neoplasms
death
Cell Death
cancer
Cells
proteins
RNA
oncogenes
Autophagy
Energy Metabolism
metabolism
Neoplasms
Proto-Oncogene Proteins c-myc
Trypan Blue
Oncogene Proteins
Polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Takai, Tomoaki ; Yoshikawa, Yuki ; Inamoto, Teruo ; Minami, Koichiro ; Taniguchi, Kohei ; Sugito, Nobuhiko ; Kuranaga, Yuki ; Shinohara, Haruka ; Kumazaki, Minami ; Tsujino, Takuya ; Takahara, Kiyoshi ; Ito, Yuko ; Akao, Yukihiro ; Azuma, Haruhito. / A novel combination RNAi toward warburg effect by replacement with miR-145 and silencing of PTBP1 induces apoptotic cell death in bladder cancer cells. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 1.
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Takai, T, Yoshikawa, Y, Inamoto, T, Minami, K, Taniguchi, K, Sugito, N, Kuranaga, Y, Shinohara, H, Kumazaki, M, Tsujino, T, Takahara, K, Ito, Y, Akao, Y & Azuma, H 2017, 'A novel combination RNAi toward warburg effect by replacement with miR-145 and silencing of PTBP1 induces apoptotic cell death in bladder cancer cells', International Journal of Molecular Sciences, vol. 18, no. 1, 179. https://doi.org/10.3390/ijms18010179

A novel combination RNAi toward warburg effect by replacement with miR-145 and silencing of PTBP1 induces apoptotic cell death in bladder cancer cells. / Takai, Tomoaki; Yoshikawa, Yuki; Inamoto, Teruo; Minami, Koichiro; Taniguchi, Kohei; Sugito, Nobuhiko; Kuranaga, Yuki; Shinohara, Haruka; Kumazaki, Minami; Tsujino, Takuya; Takahara, Kiyoshi; Ito, Yuko; Akao, Yukihiro; Azuma, Haruhito.

In: International Journal of Molecular Sciences, Vol. 18, No. 1, 179, 17.01.2017.

Research output: Contribution to journalArticle

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T1 - A novel combination RNAi toward warburg effect by replacement with miR-145 and silencing of PTBP1 induces apoptotic cell death in bladder cancer cells

AU - Takai, Tomoaki

AU - Yoshikawa, Yuki

AU - Inamoto, Teruo

AU - Minami, Koichiro

AU - Taniguchi, Kohei

AU - Sugito, Nobuhiko

AU - Kuranaga, Yuki

AU - Shinohara, Haruka

AU - Kumazaki, Minami

AU - Tsujino, Takuya

AU - Takahara, Kiyoshi

AU - Ito, Yuko

AU - Akao, Yukihiro

AU - Azuma, Haruhito

PY - 2017/1/17

Y1 - 2017/1/17

N2 - Bladder cancer is one of the most difficult malignancies to control. We explored the use of a novel RNA-interference method for a driver oncogene regulating cancer specific energy metabolism by the combination treatment with a small interfering RNA (siRNA) and a microRNA. After transfection of T24 and 253JB-V cells with miR-145 and/or siR-PTBP1, we examined the effects of cell growth and gene expression by performing the trypan blue dye exclusion test, Western blot, Hoechst 33342 staining, reverse transcription polymerase chain reaction (RT-PCR), and electron microscopy. The anti-cancer effects of xenograft model mice with miR-145 and/or siR-PTBP1 were then assessed. The combination treatment induced the deeper and longer growth inhibition and reduced the levels of both mRNA and protein expression of c-Myc and polypyrimidine tract-binding protein 1 (PTBP1) more than each single treatment. Notably, the combination treatment not only impaired the cancer specific energy metabolism by inhibiting c-Myc/PTBP1/PKMs axis but also inactivated MAPK/ERK and PI3K/AKT pathways examined in vitro and in vivo. Furthermore, the combination treatment induced apoptosis or autophagy; but, in some cells, apoptotic cell death was accompanied by autophagy, because the condensation of chromatin and many autophagosomes were coexistent. This combination treatment could be a novel RNA-interference strategy through the systemic silencing of the Warburg effect-promoting driver oncogene PTBP1 in bladder cancer cells.

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