A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size

Aekkachai Puseenam, Yasuhide Yoshioka, Rika Nagai, Reina Hashimoto, Osamu Suyari, Masanobu Itoh, Atsushi Enomoto, Masahide Takahashi, Masamitsu Yamaguchi

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


The Akt signaling pathway is well known to regulate cell proliferation and growth. Girdin, a novel substrate of Akt, plays a crucial role in organization of the actin cytoskeleton and cell motility under the control of Akt. We here identified a novel Girdin-like protein in Drosophila (dGirdin), which has two isoforms, dGirdin PA and dGirdin PB. dGirdin shows high homology with human Girdin in the N-terminal and coiled-coil domains, while diverging at the C-terminal domain. On establishment of transgenic fly lines, featuring knockdown or overexpression of dGirdin in vivo, overexpression in the wing disc cells induced ectopic apoptosis, implying a role in directing apoptosis. Knockdown of dGirdin in the Drosophila wing imaginal disc cells resulted in reduction of cell size. Furthermore, this was enhanced by half reduction of the Akt gene dose, suggesting that Akt positively regulates dGirdin. In the wing disc, cells in which dGirdin was knocked down exhibited disruption of actin filaments. From these in vivo analyses, we conclude that dGirdin is required for actin organization and regulation of appropriate cell size under control of the Akt signaling pathway.

Original languageEnglish
Pages (from-to)3370-3380
Number of pages11
JournalExperimental Cell Research
Issue number19
Publication statusPublished - 15-11-2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology


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