A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1

Chikage Suzuki, Yoshiki Murakumo, Yukari Kawase, Tomoko Sato, Takatoshi Morinaga, Naoyuki Fukuda, Atsushi Enomoto, Masatoshi Ichihara, Masahide Takahashi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Krüppel-associated box (KRAB)-containing zinc finger proteins (ZFPs) comprise the largest family of zinc finger transcription factors that function as transcriptional repressors. In the study of glial cell line-derived neurotrophic factor (GDNF)-RET signaling, we have identified bone marrow zinc finger 3 (BMZF3), encoding a KRAB-ZFP, as a GDNF-inducible gene by differential display analysis. The expression of BMZF3 transcripts in the human neuroblastoma cell line TGW increased 1 h after GDNF stimulation, as determined by Northern blotting and quantitative reverse-transcriptase polymerase chain reaction. The BMZF3 possesses transcriptional repressor activity in the KRAB domain. BMZF3 interacts with a co-repressor protein, KRAB-associated protein 1 (KAP-1), through the KRAB domain and siRNA-mediated knockdown of KAP-1 abolished the transcriptional repressor activity of BMZF3, indicating that KAP-1 is necessary for BMZF3 function. Furthermore, siRNA-mediated silencing of BMZF3 inhibited cell proliferation. These findings suggest that BMZF3 is a transcriptional repressor induced by GDNF that plays a role in cell proliferation.

Original languageEnglish
Pages (from-to)226-232
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number1
DOIs
Publication statusPublished - 01-02-2008

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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