TY - JOUR
T1 - A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1
AU - Suzuki, Chikage
AU - Murakumo, Yoshiki
AU - Kawase, Yukari
AU - Sato, Tomoko
AU - Morinaga, Takatoshi
AU - Fukuda, Naoyuki
AU - Enomoto, Atsushi
AU - Ichihara, Masatoshi
AU - Takahashi, Masahide
N1 - Funding Information:
We thank Mr. Y. Imaizumi, Mr. K. Uchiyama, and Miss S. Kawai for their technical assistance. This work was supported by Grants-in-Aid for the 21st Century Center of Excellence (COE) Research, Scientific Research (A), and Scientific Research on Priority Area ‘Cancer’ (to M.T.) and Scientific Research (C) (to Y.M.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2008/2/1
Y1 - 2008/2/1
N2 - The Krüppel-associated box (KRAB)-containing zinc finger proteins (ZFPs) comprise the largest family of zinc finger transcription factors that function as transcriptional repressors. In the study of glial cell line-derived neurotrophic factor (GDNF)-RET signaling, we have identified bone marrow zinc finger 3 (BMZF3), encoding a KRAB-ZFP, as a GDNF-inducible gene by differential display analysis. The expression of BMZF3 transcripts in the human neuroblastoma cell line TGW increased 1 h after GDNF stimulation, as determined by Northern blotting and quantitative reverse-transcriptase polymerase chain reaction. The BMZF3 possesses transcriptional repressor activity in the KRAB domain. BMZF3 interacts with a co-repressor protein, KRAB-associated protein 1 (KAP-1), through the KRAB domain and siRNA-mediated knockdown of KAP-1 abolished the transcriptional repressor activity of BMZF3, indicating that KAP-1 is necessary for BMZF3 function. Furthermore, siRNA-mediated silencing of BMZF3 inhibited cell proliferation. These findings suggest that BMZF3 is a transcriptional repressor induced by GDNF that plays a role in cell proliferation.
AB - The Krüppel-associated box (KRAB)-containing zinc finger proteins (ZFPs) comprise the largest family of zinc finger transcription factors that function as transcriptional repressors. In the study of glial cell line-derived neurotrophic factor (GDNF)-RET signaling, we have identified bone marrow zinc finger 3 (BMZF3), encoding a KRAB-ZFP, as a GDNF-inducible gene by differential display analysis. The expression of BMZF3 transcripts in the human neuroblastoma cell line TGW increased 1 h after GDNF stimulation, as determined by Northern blotting and quantitative reverse-transcriptase polymerase chain reaction. The BMZF3 possesses transcriptional repressor activity in the KRAB domain. BMZF3 interacts with a co-repressor protein, KRAB-associated protein 1 (KAP-1), through the KRAB domain and siRNA-mediated knockdown of KAP-1 abolished the transcriptional repressor activity of BMZF3, indicating that KAP-1 is necessary for BMZF3 function. Furthermore, siRNA-mediated silencing of BMZF3 inhibited cell proliferation. These findings suggest that BMZF3 is a transcriptional repressor induced by GDNF that plays a role in cell proliferation.
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U2 - 10.1016/j.bbrc.2007.11.118
DO - 10.1016/j.bbrc.2007.11.118
M3 - Article
C2 - 18060868
AN - SCOPUS:37549070367
SN - 0006-291X
VL - 366
SP - 226
EP - 232
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -