TY - JOUR
T1 - A novel heterotetrameric structure of the crenarchaeal PCNA2-PCNA3 complex
AU - Kawai, Akito
AU - Hashimoto, Hiroshi
AU - Higuchi, Shigesada
AU - Tsunoda, Masaru
AU - Sato, Mamoru
AU - Nakamura, Kazuo T.
AU - Miyamoto, Shuichi
N1 - Funding Information:
We are grateful to Dr. Eiki Yamashita, Professor Atsushi Nakagawa, and the beamline staff for their support at the BL44XU in the SPring-8. Synchrotron experiments were performed with the approval of the Joint Research Committee of the Institute for Protein Research, Osaka University, and the Japan Synchrotron Radiation Research Institute (Proposal No. 2008B6831). This work was partly supported by the National Project on Protein Structural and Functional Analysis by the Ministry of Education, Culture, Sports, Science and Technology of Japan (Protein 3000 project). We thank Professor Seiki Kuramitsu for organizing the research group in the program.
PY - 2011/6
Y1 - 2011/6
N2 - Proliferating cell nuclear antigen (PCNA) is a key protein that orchestrates the arrangement of DNA-processing proteins on DNA during DNA metabolism. In crenarchaea, PCNA forms a heterotrimer (PCNA123) consisting of PCNA1, PCNA2, and PCNA3, while in most eukaryotes and many archaea PCNAs form a homotrimer. Interestingly, unique oligomeric PCNAs from Sulfolobus tokodaii were reported in which PCNA2 and PCNA3 form a heterotrimer without PCNA1. In this paper, we describe the crystal structure of the stoPCNA2- stoPCNA3 complex. While most DNA sliding clamps form ring-shaped structures, our crystal structure showed an elliptic ring-like heterotetrameric complex, differing from a previous reports. Furthermore, we investigated the composition and the dimension of the stoPCNA2- stoPCNA3 complex in the solution using gel-filtration column chromatography and small-angle X-ray scattering analyses, respectively. These results indicate that stoPCNA2 and stoPCNA3 form the heterotetramer in solution. Based on our heterotetrameric structure, we propose a possible biological role for the heterotetrameric complex as a Holliday junction clamp.
AB - Proliferating cell nuclear antigen (PCNA) is a key protein that orchestrates the arrangement of DNA-processing proteins on DNA during DNA metabolism. In crenarchaea, PCNA forms a heterotrimer (PCNA123) consisting of PCNA1, PCNA2, and PCNA3, while in most eukaryotes and many archaea PCNAs form a homotrimer. Interestingly, unique oligomeric PCNAs from Sulfolobus tokodaii were reported in which PCNA2 and PCNA3 form a heterotrimer without PCNA1. In this paper, we describe the crystal structure of the stoPCNA2- stoPCNA3 complex. While most DNA sliding clamps form ring-shaped structures, our crystal structure showed an elliptic ring-like heterotetrameric complex, differing from a previous reports. Furthermore, we investigated the composition and the dimension of the stoPCNA2- stoPCNA3 complex in the solution using gel-filtration column chromatography and small-angle X-ray scattering analyses, respectively. These results indicate that stoPCNA2 and stoPCNA3 form the heterotetramer in solution. Based on our heterotetrameric structure, we propose a possible biological role for the heterotetrameric complex as a Holliday junction clamp.
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U2 - 10.1016/j.jsb.2011.02.006
DO - 10.1016/j.jsb.2011.02.006
M3 - Article
C2 - 21352919
AN - SCOPUS:79955672222
SN - 1047-8477
VL - 174
SP - 443
EP - 450
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 3
ER -