A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique

Kouichi Ohta; Nobuo Araki; Mamoru Shibata; Junichi Hamada; Satoru Komatsumoto; Kunio Shimazu; Yasuo Fukuuchi

doi:10.1016/0304-3940(94)90073-6

A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique

Kouichi Ohta
, Nobuo Araki
, Mamoru Shibata
, Junichi Hamada
, Satoru Komatsumoto
, Kunio Shimazu
, Yasuo Fukuuchi

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

Abstract

A novel spectrophotometric nitrite (NO^-₂) nitrate (NO^-₃) assay system for a small quantity (5 μl) of dialysate sample obtained by in vivo brain microdialysis was developed based on the diazotization reaction. The system has the advantages of in vivo consecutive measurement, high precision, good reproducibility, technical simplicity, relatively short resolution time (2.5-20 min), and wide availability. The NO^-₃ level in the rat striatum was found to be 3 times higher than the NO^-₂ level. A nitric oxide (NO) synthase inhibitor, N^G-nitro-l-arginine methyl ester, reduced striatal NO^-₂ citrulline formation in a dose-related manner and increased arginine, indicating that the tissue NO^-₂ level detected by this assay system adequately reflects the striatal NO synthase activity.

Original language	English
Pages (from-to)	165-168
Number of pages	4
Journal	Neuroscience Letters
Volume	176
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(94)90073-6
Publication status	Published - 01-08-1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/0304-3940(94)90073-6

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title = "A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique",

abstract = "A novel spectrophotometric nitrite (NO-2) nitrate (NO-3) assay system for a small quantity (5 μl) of dialysate sample obtained by in vivo brain microdialysis was developed based on the diazotization reaction. The system has the advantages of in vivo consecutive measurement, high precision, good reproducibility, technical simplicity, relatively short resolution time (2.5-20 min), and wide availability. The NO-3 level in the rat striatum was found to be 3 times higher than the NO-2 level. A nitric oxide (NO) synthase inhibitor, NG-nitro-l-arginine methyl ester, reduced striatal NO-2 citrulline formation in a dose-related manner and increased arginine, indicating that the tissue NO-2 level detected by this assay system adequately reflects the striatal NO synthase activity.",

author = "Kouichi Ohta and Nobuo Araki and Mamoru Shibata and Junichi Hamada and Satoru Komatsumoto and Kunio Shimazu and Yasuo Fukuuchi",

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T1 - A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique

AU - Ohta, Kouichi

AU - Araki, Nobuo

AU - Shibata, Mamoru

AU - Hamada, Junichi

AU - Komatsumoto, Satoru

AU - Shimazu, Kunio

AU - Fukuuchi, Yasuo

PY - 1994/8/1

Y1 - 1994/8/1

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AB - A novel spectrophotometric nitrite (NO-2) nitrate (NO-3) assay system for a small quantity (5 μl) of dialysate sample obtained by in vivo brain microdialysis was developed based on the diazotization reaction. The system has the advantages of in vivo consecutive measurement, high precision, good reproducibility, technical simplicity, relatively short resolution time (2.5-20 min), and wide availability. The NO-3 level in the rat striatum was found to be 3 times higher than the NO-2 level. A nitric oxide (NO) synthase inhibitor, NG-nitro-l-arginine methyl ester, reduced striatal NO-2 citrulline formation in a dose-related manner and increased arginine, indicating that the tissue NO-2 level detected by this assay system adequately reflects the striatal NO synthase activity.

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A novel in vivo assay system for consecutive measurement of brain nitric oxide production combined with the microdialysis technique

Abstract

All Science Journal Classification (ASJC) codes

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