TY - JOUR
T1 - A novel membrane guanylyl cyclase expressed in medaka (Oryzias latipes) intestine
AU - Iio, Kaori
AU - Nakauchi, Mina
AU - Yamagami, Sayaka
AU - Tsutsumi, Makiko
AU - Hori, Hiroshi
AU - Naruse, Kiyoshi
AU - Mitani, Hiroshi
AU - Shima, Akihiro
AU - Suzuki, Norio
N1 - Funding Information:
We are grateful to the staff members of the Center for Advanced Science and Technology, Hokkaido University, for the use of their laboratory facilities. We would also like to thank Dr. Minoru Tanaka, Hokkaido University, for the use of his laboratory facilities. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (No. 11236202) and by the National Project on Protein Structural and Functional Analyses.
PY - 2005/4
Y1 - 2005/4
N2 - A novel membrane guanylyl cyclase (GC), OlGC9, was identified in the intestine of the medaka fish Oryzias latipes by the isolation of a full-length cDNA clone (3783 bp). Phylogenetic analysis indicated that OlGC9 belongs in the enterotoxin/guanylin receptor membrane GC subfamily. The nucleotide and deduced amino acid sequences of OlGC9 were highly homologous to those of OlGC6, another enterotoxin/guanylin receptor membrane GC in medaka fish. Linkage analysis of the medaka fish chromosome demonstrated that the OlGC9 gene was mapped to LG8, which distinguishes it from the OlGC6 gene. Determination of the cGMP concentrations in COS-7 cells expressed with OlGC9 indicated that Escherichia coli heat-stable enterotoxin (STa) stimulated the activity of OlGC9 in a concentration-dependent manner, although it did not activate the OlGC6 expressed in the COS-7 cells. The 5′-flanking region of the OlGC9 gene important for its transcription was partially determined using both CACO-2 cells and COS-1 cells, and was not found to be conserved with respect to either the mammalian GC-C gene or the OlGC6 gene.
AB - A novel membrane guanylyl cyclase (GC), OlGC9, was identified in the intestine of the medaka fish Oryzias latipes by the isolation of a full-length cDNA clone (3783 bp). Phylogenetic analysis indicated that OlGC9 belongs in the enterotoxin/guanylin receptor membrane GC subfamily. The nucleotide and deduced amino acid sequences of OlGC9 were highly homologous to those of OlGC6, another enterotoxin/guanylin receptor membrane GC in medaka fish. Linkage analysis of the medaka fish chromosome demonstrated that the OlGC9 gene was mapped to LG8, which distinguishes it from the OlGC6 gene. Determination of the cGMP concentrations in COS-7 cells expressed with OlGC9 indicated that Escherichia coli heat-stable enterotoxin (STa) stimulated the activity of OlGC9 in a concentration-dependent manner, although it did not activate the OlGC6 expressed in the COS-7 cells. The 5′-flanking region of the OlGC9 gene important for its transcription was partially determined using both CACO-2 cells and COS-1 cells, and was not found to be conserved with respect to either the mammalian GC-C gene or the OlGC6 gene.
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U2 - 10.1016/j.cbpc.2004.12.006
DO - 10.1016/j.cbpc.2004.12.006
M3 - Article
C2 - 15763512
AN - SCOPUS:14844321877
SN - 1096-4959
VL - 140
SP - 569
EP - 578
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 4
ER -