TY - JOUR
T1 - A novel method for measuring serum ornithine carbamoyltransferase
AU - Ishikawa, Hiroaki
AU - Matsuzawa, Takeo
AU - Ohashi, Koji
AU - Nagamura, Yoichi
PY - 2003/5
Y1 - 2003/5
N2 - Background: Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. Methods: We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, using ornithine-ketoacid aminotransferase, Δ1-pyrroline-5-carboxylate dehydrogenase and glutamate dehydrogenase, which together convert citrulline through ornithine to glutamate. The glutamate is then quantitatively measured using glutamate oxidase and Trinder's reagent. Results: The results obtained by this method agreed well with those obtained using the diacetylmonoxime method as a gold standard [correlation coefficient (r) = 0.973 P<0.001]. The endogenous amino acids sensitive to this method in serum (glutamate, ornithine and Δ1-pyrroline-5-carboxylate) were eliminated by the initial futile reaction. The new method appears to be more accurate at low levels of ornithine carbamoyltransferase activity than the diacetylmonoxime method. Conclusions: Here we report a new method for serum ornithine carbamoyltransferase assay which might be useful for clinical diagnosis of hepatic disorders, including hepatic cancer.
AB - Background: Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. Methods: We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, using ornithine-ketoacid aminotransferase, Δ1-pyrroline-5-carboxylate dehydrogenase and glutamate dehydrogenase, which together convert citrulline through ornithine to glutamate. The glutamate is then quantitatively measured using glutamate oxidase and Trinder's reagent. Results: The results obtained by this method agreed well with those obtained using the diacetylmonoxime method as a gold standard [correlation coefficient (r) = 0.973 P<0.001]. The endogenous amino acids sensitive to this method in serum (glutamate, ornithine and Δ1-pyrroline-5-carboxylate) were eliminated by the initial futile reaction. The new method appears to be more accurate at low levels of ornithine carbamoyltransferase activity than the diacetylmonoxime method. Conclusions: Here we report a new method for serum ornithine carbamoyltransferase assay which might be useful for clinical diagnosis of hepatic disorders, including hepatic cancer.
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U2 - 10.1258/000456303321610583
DO - 10.1258/000456303321610583
M3 - Article
C2 - 12803840
AN - SCOPUS:0037690421
SN - 0004-5632
VL - 40
SP - 264
EP - 268
JO - Annals of Clinical Biochemistry
JF - Annals of Clinical Biochemistry
IS - 3
ER -