A novel method for measuring serum ornithine carbamoyltransferase

Hiroaki Ishikawa, Takeo Matsuzawa, Koji Ohashi, Yoichi Nagamura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. Methods: We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, using ornithine-ketoacid aminotransferase, Δ1-pyrroline-5-carboxylate dehydrogenase and glutamate dehydrogenase, which together convert citrulline through ornithine to glutamate. The glutamate is then quantitatively measured using glutamate oxidase and Trinder's reagent. Results: The results obtained by this method agreed well with those obtained using the diacetylmonoxime method as a gold standard [correlation coefficient (r) = 0.973 P<0.001]. The endogenous amino acids sensitive to this method in serum (glutamate, ornithine and Δ1-pyrroline-5-carboxylate) were eliminated by the initial futile reaction. The new method appears to be more accurate at low levels of ornithine carbamoyltransferase activity than the diacetylmonoxime method. Conclusions: Here we report a new method for serum ornithine carbamoyltransferase assay which might be useful for clinical diagnosis of hepatic disorders, including hepatic cancer.

Original languageEnglish
Pages (from-to)264-268
Number of pages5
JournalAnnals of Clinical Biochemistry
Volume40
Issue number3
DOIs
Publication statusPublished - 01-05-2003

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Ornithine Carbamoyltransferase
Glutamic Acid
Serum
Ornithine
1-Pyrroline-5-Carboxylate Dehydrogenase
Ornithine-Oxo-Acid Transaminase
Glutamate Dehydrogenase
Citrulline
Mitochondria
Assays
Oxidoreductases
Liver
Liver Neoplasms
Amino Acids

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

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abstract = "Background: Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. Methods: We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, using ornithine-ketoacid aminotransferase, Δ1-pyrroline-5-carboxylate dehydrogenase and glutamate dehydrogenase, which together convert citrulline through ornithine to glutamate. The glutamate is then quantitatively measured using glutamate oxidase and Trinder's reagent. Results: The results obtained by this method agreed well with those obtained using the diacetylmonoxime method as a gold standard [correlation coefficient (r) = 0.973 P<0.001]. The endogenous amino acids sensitive to this method in serum (glutamate, ornithine and Δ1-pyrroline-5-carboxylate) were eliminated by the initial futile reaction. The new method appears to be more accurate at low levels of ornithine carbamoyltransferase activity than the diacetylmonoxime method. Conclusions: Here we report a new method for serum ornithine carbamoyltransferase assay which might be useful for clinical diagnosis of hepatic disorders, including hepatic cancer.",
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A novel method for measuring serum ornithine carbamoyltransferase. / Ishikawa, Hiroaki; Matsuzawa, Takeo; Ohashi, Koji; Nagamura, Yoichi.

In: Annals of Clinical Biochemistry, Vol. 40, No. 3, 01.05.2003, p. 264-268.

Research output: Contribution to journalArticle

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