A novel molecule 'shati' increases dopamine uptake via the induction of tumor necrosis factor-α in pheochromocytoma-12 cells

Minae Niwa, Atsumi Nitta, Xiaobo Cen, Kiyoyuki Kitaichi, Norio Ozaki, Kiyofumi Yamada, Toshitaka Nabeshima

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The psychostimulant properties of methamphetamine (METH) are associated with an increase in extracellular dopamine (DA) levels in the brain, via facilitation of DA's release from pre-synaptic nerve terminals and inhibition of its reuptake through DA transporter. Recently, we have demonstrated that tumor necrosis factor-α (TNF-α) increases DA uptake and inhibits METH dependence. Moreover, we have clarified 'shati' identified in the nucleus accumbens of mice treated with METH is involved in METH dependence. In the present study, we investigated the effects of TNF-α on DA uptake in PC12 cells and established a PC12 cell line transfected with a vector containing shati cDNA to examine the precise mechanism behind the role of shati in DA uptake. Moreover, we examined the relationship between shati and TNF-α. TNF-α increased DA uptake via the mitogen-activated protein kinase kinase pathway and inhibited the METH-induced decrease in DA uptake in PC12 cells. Transfection of the vector containing shati cDNA into PC12 cells, induced the expression of shati and TNF-α mRNA, accelerated DA uptake, and inhibited the METH-induced decrease in DA uptake. These results suggest that the functional roles of shati in METH-regulated behavioral changes are mediated through inhibition of the METH-induced decrease in DA uptake via TNF-α.

Original languageEnglish
Pages (from-to)1697-1708
Number of pages12
JournalJournal of Neurochemistry
Volume107
Issue number6
DOIs
Publication statusPublished - 01-12-2008
Externally publishedYes

Fingerprint

Pheochromocytoma
Methamphetamine
Dopamine
Tumor Necrosis Factor-alpha
Molecules
PC12 Cells
Complementary DNA
Dopamine Plasma Membrane Transport Proteins
Mitogen-Activated Protein Kinase Kinases
Presynaptic Terminals
Nucleus Accumbens
Transfection
Brain
Cells
Cell Line
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Niwa, Minae ; Nitta, Atsumi ; Cen, Xiaobo ; Kitaichi, Kiyoyuki ; Ozaki, Norio ; Yamada, Kiyofumi ; Nabeshima, Toshitaka. / A novel molecule 'shati' increases dopamine uptake via the induction of tumor necrosis factor-α in pheochromocytoma-12 cells. In: Journal of Neurochemistry. 2008 ; Vol. 107, No. 6. pp. 1697-1708.
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A novel molecule 'shati' increases dopamine uptake via the induction of tumor necrosis factor-α in pheochromocytoma-12 cells. / Niwa, Minae; Nitta, Atsumi; Cen, Xiaobo; Kitaichi, Kiyoyuki; Ozaki, Norio; Yamada, Kiyofumi; Nabeshima, Toshitaka.

In: Journal of Neurochemistry, Vol. 107, No. 6, 01.12.2008, p. 1697-1708.

Research output: Contribution to journalArticle

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