A novel monoclonal antibody against rodent common Thy- 1.1 Epitope

Miyuki Kinebuchi, Akihiro Matsuura, Toshimitu Uede, Yoshifumi Ishii, Kokichi Kikuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Here we report a new anti-rat Thy-1 monoclonal antibody (MAb) 12C5 that recognized a novel epitope on a GPI-anchored Thy-1 antigen. In rat thymocytes, MAb 12C5 reacted with 25 kDa Thy-1 antigen purified with MAb OX7. In rats, MAb 12C5 was expressed on 94% of thymocytes, 46% of spleen cells, 31% of mesenteric lymphnode cells, 76% of bone marrow cells, and 24% of splenic T cells. Furthermore, MAb 12C5 reacted with the hematopoietic cells in Mongolian gerbils, hamsters, and AKR mice (Thy-1.1) as in rats. In AKR mice, almost all bone marrow cells reacted with MAb 12C5. Thus, MAb 12C5 recognized a novel epitope of Thy-1 that was widespread in hematopoietic cells of rats and mice, as well as other rodents.

Original languageEnglish
Pages (from-to)457-462
Number of pages6
JournalHybridoma
Volume17
Issue number5
DOIs
Publication statusPublished - 01-01-1998
Externally publishedYes

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Epitopes
Rodentia
Monoclonal Antibodies
Thy-1 Antigens
Inbred AKR Mouse
Thymocytes
Bone Marrow Cells
Gerbillinae
Cricetinae
Spleen
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Kinebuchi, Miyuki ; Matsuura, Akihiro ; Uede, Toshimitu ; Ishii, Yoshifumi ; Kikuchi, Kokichi. / A novel monoclonal antibody against rodent common Thy- 1.1 Epitope. In: Hybridoma. 1998 ; Vol. 17, No. 5. pp. 457-462.
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Kinebuchi, M, Matsuura, A, Uede, T, Ishii, Y & Kikuchi, K 1998, 'A novel monoclonal antibody against rodent common Thy- 1.1 Epitope', Hybridoma, vol. 17, no. 5, pp. 457-462. https://doi.org/10.1089/hyb.1998.17.457

A novel monoclonal antibody against rodent common Thy- 1.1 Epitope. / Kinebuchi, Miyuki; Matsuura, Akihiro; Uede, Toshimitu; Ishii, Yoshifumi; Kikuchi, Kokichi.

In: Hybridoma, Vol. 17, No. 5, 01.01.1998, p. 457-462.

Research output: Contribution to journalArticle

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AU - Kinebuchi, Miyuki

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