TY - JOUR
T1 - A novel nuclear phosphoprotein, GANP, is up-regulated in centrocytes of the germinal center and associated with MCM3, a protein essential for DNA replication
AU - Kuwahara, Kazuhiko
AU - Yoshida, Mikoto
AU - Kondo, Eisaku
AU - Sakata, Atsuko
AU - Watanabe, Yuko
AU - Abe, Eiji
AU - Kouno, Yoshihiko
AU - Tomiyasu, Shinjirou
AU - Fujimura, Satoru
AU - Tokuhisa, Takeshi
AU - Kimura, Hiroshi
AU - Ezaki, Taichi
AU - Sakaguchi, Nobuo
PY - 2000/4/1
Y1 - 2000/4/1
N2 - Antigen (Ag) immunization induces formation of the germinal center (GC), with large, rapidly proliferating centroblasts in the dark zone, and small, nondividing centrocytes in the light zone. We identified a novel nuclear protein, GANP, that is up-regulated in centrocytes. We found that GANP was up-regulated in GC B cells of Peyer's patches in normal mice and in spleens from Ag-Immunized mice. GANP-positive cells appeared in the light zone of the GC, with coexpression of the peanut agglutinin (PNA) (PNA)-positive B220- positive phenotype. The expression of GANP was strikingly correlated with GC formation because Bcl6-deficient mice did not show the up-regulation of GANP. GANP-positive cells were mostly surrounded by follicular dendritic cells. Stimulation with anti-μ and anti-CD40 induced up-regulation of ganp messenger RNA as well as GANP protein in B220-positive B cells in vitro. GANP is a 210-kd protein localized in both the cytoplasm and nuclei, with a homologous region to Map80 that is associated with MCM3, a protein essential for DNA replication. Remarkably, GANP is associated with MCM3 in B cells and MCM3 is also up-regulated in the GC area. These results suggest that the up- regulation of GANP might participate in the development of Ag-driven B cells in GCs through its interaction with MCM3. (C) 2000 by The American Society of Hematology.
AB - Antigen (Ag) immunization induces formation of the germinal center (GC), with large, rapidly proliferating centroblasts in the dark zone, and small, nondividing centrocytes in the light zone. We identified a novel nuclear protein, GANP, that is up-regulated in centrocytes. We found that GANP was up-regulated in GC B cells of Peyer's patches in normal mice and in spleens from Ag-Immunized mice. GANP-positive cells appeared in the light zone of the GC, with coexpression of the peanut agglutinin (PNA) (PNA)-positive B220- positive phenotype. The expression of GANP was strikingly correlated with GC formation because Bcl6-deficient mice did not show the up-regulation of GANP. GANP-positive cells were mostly surrounded by follicular dendritic cells. Stimulation with anti-μ and anti-CD40 induced up-regulation of ganp messenger RNA as well as GANP protein in B220-positive B cells in vitro. GANP is a 210-kd protein localized in both the cytoplasm and nuclei, with a homologous region to Map80 that is associated with MCM3, a protein essential for DNA replication. Remarkably, GANP is associated with MCM3 in B cells and MCM3 is also up-regulated in the GC area. These results suggest that the up- regulation of GANP might participate in the development of Ag-driven B cells in GCs through its interaction with MCM3. (C) 2000 by The American Society of Hematology.
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U2 - 10.1182/blood.v95.7.2321.007k33_2321_2328
DO - 10.1182/blood.v95.7.2321.007k33_2321_2328
M3 - Article
C2 - 10733502
AN - SCOPUS:12944266918
VL - 95
SP - 2321
EP - 2328
JO - Blood
JF - Blood
SN - 0006-4971
IS - 7
ER -