A novel rat orthologue and homologue for the Drosophila crooked neck gene in neural stem cells and their immediate descendants

Naoki Amada, Tomoyuki Tezuka, Akira Maeda, Kazuaki Araki, Nobuyuki Takei, Kazuo Todokoro, Hiroyuki Nawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The crooked neck (crn) gene of Drosophila melanogaster encodes a scaffold protein carrying multiple tetratricopeptide repeat (TPR) motifs, and its mutation results in a reduction in the number of neuroblasts and lethality during larval stages. Here, we isolated two structurally related genes from a rat embryonic brain cDNA library. One gene is the rat orthologue of crn, which encodes 690 amino acids including 16 copies of TPR. The other gene, ATH55, encodes an 855 amino acid protein including 21 TPR motifs, which presumably represents a rat crn homologue and an orthologue of human XAB2. Both genes are highly expressed in embryonic brain but their expressions decrease during development. ATH55-like immunoreactivity is present in the ventricular zone and newly formed cortical plate, while CRN-like immunoreactivity is more abundant in a younger ventricular zone. In agreement, both proteins were found to be enriched in cultured neural stem cells and to decrease in response to cell differentiation signals. As indicated for the yeast CRN-like protein, ATH55 and CRN immunoreactivities were both recovered in the nuclear fraction and detected in the splicing complex carrying pre-mRNA. These findings suggest that both TPR-motif-containing proteins are involved in RNA processing of mammalian neural stem cells and their immediate descendants.

Original languageEnglish
Pages (from-to)615-623
Number of pages9
JournalJournal of Biochemistry
Volume133
Issue number5
DOIs
Publication statusPublished - 01-05-2003

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Neural Stem Cells
Stem cells
Drosophila
Rats
Neck
Genes
Proteins
Brain
Amino Acids
Amino Acid Motifs
RNA Precursors
Drosophila melanogaster
Gene Library
Scaffolds
Cerebral Cortex
Yeast
Cell Differentiation
Yeasts
RNA
Mutation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Amada, Naoki ; Tezuka, Tomoyuki ; Maeda, Akira ; Araki, Kazuaki ; Takei, Nobuyuki ; Todokoro, Kazuo ; Nawa, Hiroyuki. / A novel rat orthologue and homologue for the Drosophila crooked neck gene in neural stem cells and their immediate descendants. In: Journal of Biochemistry. 2003 ; Vol. 133, No. 5. pp. 615-623.
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A novel rat orthologue and homologue for the Drosophila crooked neck gene in neural stem cells and their immediate descendants. / Amada, Naoki; Tezuka, Tomoyuki; Maeda, Akira; Araki, Kazuaki; Takei, Nobuyuki; Todokoro, Kazuo; Nawa, Hiroyuki.

In: Journal of Biochemistry, Vol. 133, No. 5, 01.05.2003, p. 615-623.

Research output: Contribution to journalArticle

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