A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting

Kazuto Kobayashi, Tamae Oe, Ira Pastan, Toshiharu Nagatsu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor α subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.

Original languageEnglish
Pages (from-to)3653-3655
Number of pages3
JournalNucleic Acids Research
Volume24
Issue number18
Publication statusPublished - 01-10-1996

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Immunotoxins
Embryonic Stem Cells
Interleukin-2 Receptors
Clone Cells
Mutation
Mouse Embryonic Stem Cells

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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abstract = "Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor α subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.",
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A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting. / Kobayashi, Kazuto; Oe, Tamae; Pastan, Ira; Nagatsu, Toshiharu.

In: Nucleic Acids Research, Vol. 24, No. 18, 01.10.1996, p. 3653-3655.

Research output: Contribution to journalArticle

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