A PKC-mediated backup mechanism of the MXXCW motif-linked switch for initiating tyrosine kinase activities

Kozue Takeda, Yoshiyuki Kawamoto, Yusuke Okuno, Masashi Kato, Masahide Takahashi, Haruhiko Suzuki, Kenichi Isobe, Izumi Nakashima

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The cysteine in the M/IXXCW motif is conserved in all but one (threonine in place of cysteine) of the human protein tyrosine kinases (PTKs). We showed that all RET-PTC-1 mutants in which the C in this motif (C376) was replaced with glycine, lysine, threonine or serine lost their activity in vitro. However, the C376T/S mutants showed normal tyrosine phosphorylation in vivo (in cells). Further analyses reveled that protein kinase C (PKC) initiated the activities of the C376T/S mutants in cells. We conclude that the M/IXXCW motif-mediated mechanisms which initiate PTK activities are partially replaced by a PKC-mediated mechanism.

Original languageEnglish
Pages (from-to)839-843
Number of pages5
JournalFEBS Letters
Volume580
Issue number3
DOIs
Publication statusPublished - 06-02-2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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