TY - JOUR
T1 - A PKC-mediated backup mechanism of the MXXCW motif-linked switch for initiating tyrosine kinase activities
AU - Takeda, Kozue
AU - Kawamoto, Yoshiyuki
AU - Okuno, Yusuke
AU - Kato, Masashi
AU - Takahashi, Masahide
AU - Suzuki, Haruhiko
AU - Isobe, Kenichi
AU - Nakashima, Izumi
N1 - Funding Information:
We thank M. Nagao and Y. Ishizaka for providing us with RET-PTC-1 cDNA. This study was supported in part by Grants-in-Aid for Scientific Research B and Scientific Research of Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2006/2/6
Y1 - 2006/2/6
N2 - The cysteine in the M/IXXCW motif is conserved in all but one (threonine in place of cysteine) of the human protein tyrosine kinases (PTKs). We showed that all RET-PTC-1 mutants in which the C in this motif (C376) was replaced with glycine, lysine, threonine or serine lost their activity in vitro. However, the C376T/S mutants showed normal tyrosine phosphorylation in vivo (in cells). Further analyses reveled that protein kinase C (PKC) initiated the activities of the C376T/S mutants in cells. We conclude that the M/IXXCW motif-mediated mechanisms which initiate PTK activities are partially replaced by a PKC-mediated mechanism.
AB - The cysteine in the M/IXXCW motif is conserved in all but one (threonine in place of cysteine) of the human protein tyrosine kinases (PTKs). We showed that all RET-PTC-1 mutants in which the C in this motif (C376) was replaced with glycine, lysine, threonine or serine lost their activity in vitro. However, the C376T/S mutants showed normal tyrosine phosphorylation in vivo (in cells). Further analyses reveled that protein kinase C (PKC) initiated the activities of the C376T/S mutants in cells. We conclude that the M/IXXCW motif-mediated mechanisms which initiate PTK activities are partially replaced by a PKC-mediated mechanism.
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U2 - 10.1016/j.febslet.2006.01.005
DO - 10.1016/j.febslet.2006.01.005
M3 - Article
C2 - 16427628
AN - SCOPUS:31444451659
SN - 0014-5793
VL - 580
SP - 839
EP - 843
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -