A potent anti-angiogenic factor, vasohibin-1, ameliorates experimental bronchiolitis obliterans

T. Watanabe, Y. Okada, Yasushi Hoshikawa, S. Eba, H. Notsuda, Y. Watanabe, H. Ohishi, Y. Sato, T. Kondo

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Abstract

Background: Bronchiolitis obliterans (BO) is a major cause of morbidity and mortality after lung transplantation. BO is pathologically characterized by neovascularized fibro-obliteration of the allograft airway. A recent study has shown that aberrant angiogenesis during fibro-obliteration contributes to the pathogenesis of BO. Vasohibin-1 (VASH1) has been isolated as a vascular endothelial growth factor-inducible gene in endothelial cells (ECs) that inhibits migration and proliferation of ECs and exhibits anti-angiogenic activity in vivo. Purpose: This study examines whether VASH1 inhibits fibro-obliteration of the allograft in a murine intrapulmonary tracheal transplantation model. Method: Tracheal allografts of BALB/c mouse were transplanted into the left lung of recipient C57BL/6J mouse. We performed gene transfer to the recipient lungs using an adenovirus vector encoding human VASH1 (Ad-VASH1) or beta- garactosidase (Ad-LacZ) as the control. Tracheal allografts were harvested and pathological on days 21 and 28. Result: Ad-VASH1 treatment reduced the vascular area on day 21 (4.6% versus 13.0%, P =.037) and day 28 (5.4% versus 13.4%, P =.022) compared with the control group. This was accompanied by significantly inhibited luminal obliteration of the tracheal allografts in the animals transferred with Ad-VASH1 compared with the control (69% versus 93%, P =.028) on day 21. We were not able to observe this effect on day 28 (92% versus 97%, P =.48). Conclusion: Transgene expression of VASH1 in the recipient lung significantly attenuated luminal obliteration of the tracheal allograft; this was associated with significantly reduced aberrant angiogenesis in the fibro-obliterative tissue in a murine model intrapulmonary tracheal transplantation.

Original languageEnglish
Pages (from-to)1155-1157
Number of pages3
JournalTransplantation Proceedings
Volume44
Issue number4
DOIs
Publication statusPublished - 01-05-2012

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All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Watanabe, T., Okada, Y., Hoshikawa, Y., Eba, S., Notsuda, H., Watanabe, Y., Ohishi, H., Sato, Y., & Kondo, T. (2012). A potent anti-angiogenic factor, vasohibin-1, ameliorates experimental bronchiolitis obliterans. Transplantation Proceedings, 44(4), 1155-1157. https://doi.org/10.1016/j.transproceed.2012.02.022