A potential therapeutic system for Alzheimer's disease using adsorbents with alkyl ligands for removal of blood amyloid β

Kazunori Kawaguchi, Masato Takeuchi, Hiromasa Yamagawa, Kazutaka Murakami, Sigeru Nakai, Hideo Hori, Atsushi Ohashi, Yoshiyuki Hiki, Nobuo Suzuki, Satoshi Sugiyama, Yukio Yuzawa, Nobuya Kitaguchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Amyloid beta proteins (Aβ) in the brain are the main cause of Alzheimer's disease. Peripheral administration of Aβ-binding substances, which may act as a sink for Aβ from the brain, has been reported to reduce brain Aβ. We previously found C16-cellulose beads had high Aβ-removal activity in vitro. In this study, we investigated the optimum surface properties of adsorbents for removal of Aβ in vitro and in humans. Batch analysis was performed with porous cellulose beads or silica beads with or without 2-22 methylene groups. Aβ-removal activity of C16-cellulose beads increased with increasing alkyl chain length. In contrast, with cellulose the amount of Aβ removed by the silica beads decreased with increasing alkyl chain length. Cellulose beads with 16 or 22 methylene groups were best (over 99 % removal) among all the beads tested (p ≤ 0.01). The adsorbent surfaces were analyzed by near-infrared spectroscopy, which revealed that the optimum beads had a sufficiently hydrophobic surface with an appropriate amount of adsorbed water accessible on the surface. Aβ removal efficiency by C16-cellulose beads was investigated for 5 renal failure patients on hemodialysis, resulting in 51.1 ± 6.6 % for Aβ1-40 and 43.8 ± 4.5 % for Aβ1-42 (p ≤ 0.01). In conclusion, cellulose beads with 16 or 22 methylene groups and an appropriate amount of adsorbed water were the optimum Aβ adsorbents. The device with C16-cellulose beads had high Aβ removal activity in humans. These adsorbents might be useful for Alzheimer's disease therapy.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalJournal of Artificial Organs
Volume16
Issue number2
DOIs
Publication statusPublished - 01-06-2013

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Amyloid
Cellulose
Adsorbents
Alzheimer Disease
Blood
Ligands
Brain
Therapeutics
Chain length
Silicon Dioxide
Silica
Near infrared spectroscopy
Near-Infrared Spectroscopy
Surface Properties
Water
Amyloid beta-Peptides
Staphylococcal Protein A
Human Activities
Surface properties
Renal Insufficiency

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

Cite this

Kawaguchi, Kazunori ; Takeuchi, Masato ; Yamagawa, Hiromasa ; Murakami, Kazutaka ; Nakai, Sigeru ; Hori, Hideo ; Ohashi, Atsushi ; Hiki, Yoshiyuki ; Suzuki, Nobuo ; Sugiyama, Satoshi ; Yuzawa, Yukio ; Kitaguchi, Nobuya. / A potential therapeutic system for Alzheimer's disease using adsorbents with alkyl ligands for removal of blood amyloid β. In: Journal of Artificial Organs. 2013 ; Vol. 16, No. 2. pp. 211-217.
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A potential therapeutic system for Alzheimer's disease using adsorbents with alkyl ligands for removal of blood amyloid β. / Kawaguchi, Kazunori; Takeuchi, Masato; Yamagawa, Hiromasa; Murakami, Kazutaka; Nakai, Sigeru; Hori, Hideo; Ohashi, Atsushi; Hiki, Yoshiyuki; Suzuki, Nobuo; Sugiyama, Satoshi; Yuzawa, Yukio; Kitaguchi, Nobuya.

In: Journal of Artificial Organs, Vol. 16, No. 2, 01.06.2013, p. 211-217.

Research output: Contribution to journalArticle

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T1 - A potential therapeutic system for Alzheimer's disease using adsorbents with alkyl ligands for removal of blood amyloid β

AU - Kawaguchi, Kazunori

AU - Takeuchi, Masato

AU - Yamagawa, Hiromasa

AU - Murakami, Kazutaka

AU - Nakai, Sigeru

AU - Hori, Hideo

AU - Ohashi, Atsushi

AU - Hiki, Yoshiyuki

AU - Suzuki, Nobuo

AU - Sugiyama, Satoshi

AU - Yuzawa, Yukio

AU - Kitaguchi, Nobuya

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