A practical method for phenotypic detection of metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter spp. using sulfamoyl heteroarylcarboxylic acids

Chihiro Norizuki; Yoshichika Arakawa; Jun ichi Wachino

doi:10.1016/j.mimet.2025.107235

A practical method for phenotypic detection of metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter spp. using sulfamoyl heteroarylcarboxylic acids

Chihiro Norizuki
, Yoshichika Arakawa
, Jun ichi Wachino

Clinical Microbiology and Infection

Research output: Contribution to journal › Article › peer-review

Abstract

Phenotypic screening using subclass B1 metallo-β-lactamase (MBL)-specific sulfamoyl heteroarylcarboxylic acid inhibitors (2,5-dimethyl-4-sulfamoylfuran-3-carboxylic acid and 2,5-diethyl-1-methyl-4-sulfamoylpyrrole-3-carboxylic acid) showed high sensitivity and specificity for detecting MBL-producing Pseudomonas aeruginosa and Acinetobacter spp. This practical and cost-effective method may support routine detection of MBLs in clinical laboratories lacking access to molecular diagnostic tools.

Original language	English
Article number	107235
Journal	Journal of Microbiological Methods
Volume	237
DOIs	https://doi.org/10.1016/j.mimet.2025.107235
Publication status	Published - 10-2025

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2025.107235

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title = "A practical method for phenotypic detection of metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter spp. using sulfamoyl heteroarylcarboxylic acids",

abstract = "Phenotypic screening using subclass B1 metallo-β-lactamase (MBL)-specific sulfamoyl heteroarylcarboxylic acid inhibitors (2,5-dimethyl-4-sulfamoylfuran-3-carboxylic acid and 2,5-diethyl-1-methyl-4-sulfamoylpyrrole-3-carboxylic acid) showed high sensitivity and specificity for detecting MBL-producing Pseudomonas aeruginosa and Acinetobacter spp. This practical and cost-effective method may support routine detection of MBLs in clinical laboratories lacking access to molecular diagnostic tools.",

keywords = "Acinetobacter, Carbapenems, Metallo-β-lactamase, Phenotypic screening, Pseudomonas aeruginosa, Sulfamoyl heteroarylcarboxylic acid",

author = "Chihiro Norizuki and Yoshichika Arakawa and Wachino, \{Jun ichi\}",

note = "Publisher Copyright: {\textcopyright} 2025 Elsevier B.V.",

year = "2025",

month = oct,

doi = "10.1016/j.mimet.2025.107235",

language = "English",

volume = "237",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - A practical method for phenotypic detection of metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter spp. using sulfamoyl heteroarylcarboxylic acids

AU - Norizuki, Chihiro

AU - Arakawa, Yoshichika

AU - Wachino, Jun ichi

PY - 2025/10

Y1 - 2025/10

N2 - Phenotypic screening using subclass B1 metallo-β-lactamase (MBL)-specific sulfamoyl heteroarylcarboxylic acid inhibitors (2,5-dimethyl-4-sulfamoylfuran-3-carboxylic acid and 2,5-diethyl-1-methyl-4-sulfamoylpyrrole-3-carboxylic acid) showed high sensitivity and specificity for detecting MBL-producing Pseudomonas aeruginosa and Acinetobacter spp. This practical and cost-effective method may support routine detection of MBLs in clinical laboratories lacking access to molecular diagnostic tools.

AB - Phenotypic screening using subclass B1 metallo-β-lactamase (MBL)-specific sulfamoyl heteroarylcarboxylic acid inhibitors (2,5-dimethyl-4-sulfamoylfuran-3-carboxylic acid and 2,5-diethyl-1-methyl-4-sulfamoylpyrrole-3-carboxylic acid) showed high sensitivity and specificity for detecting MBL-producing Pseudomonas aeruginosa and Acinetobacter spp. This practical and cost-effective method may support routine detection of MBLs in clinical laboratories lacking access to molecular diagnostic tools.

KW - Acinetobacter

KW - Carbapenems

KW - Metallo-β-lactamase

KW - Phenotypic screening

KW - Pseudomonas aeruginosa

KW - Sulfamoyl heteroarylcarboxylic acid

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DO - 10.1016/j.mimet.2025.107235

M3 - Article

C2 - 40848750

AN - SCOPUS:105014281915

SN - 0167-7012

VL - 237

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 107235

ER -

A practical method for phenotypic detection of metallo-β-lactamase-producing Pseudomonas aeruginosa and Acinetobacter spp. using sulfamoyl heteroarylcarboxylic acids

Abstract

All Science Journal Classification (ASJC) codes

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