A simple quantitative assay for urinary adenosine using column-switching high-performance liquid chromatography

Hiroko Taniai, Satoshi Sumi, Tetsuya Ito, Akihito Ueta, Yumiko Ohkubo, Hajime Togari

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Adenosine is a physiologically active molecule produced locally in many sites of the body to regulate various cell functions. Measurement of levels of the factor in organs and biological fluids provides clues to its role and we reported an accurate quantitative high-performance liquid chromatography method for urinary adenosine requiring no preliminary sample preparation, other than filtration. Analyses were performed isocratically with a reversed-phase and a molecular exclusion columns connected by a column switch. Each sample was analyzed automatically in 35 min. Linearity could be verified up to 1,000 μmol/L (r = 0.999) and recovery of adenosine was 94.6 - 98.0%. The coefficients of variation (CV) were established to be 0.56 - 1.32%, intra-assay, and 1.61 - 4.67%, inter-assay. Based on analyses of healthy individuals at different ages, we are here able to provide age-related values, infants (1.51 ± 0.71 μmol/mmol creatinine) and children (1.06 ± 0.36 and 0.83 ± 0.27 μmol/mmol creatinine; aged 1 - 5 and 6 - 10 years), excreting significantly higher amounts of adenosine than adults (0.44 ± 0.08 μmol/mmol creatinine). We also measured urinary adenosine from patients suffering from metabolic disease or severe respiratory failure and found that unfavorable pathophysiologic conditions are associated with appreciable elevation of adenosine.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalTohoku Journal of Experimental Medicine
Volume208
Issue number1
DOIs
Publication statusPublished - 02-12-2005

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High performance liquid chromatography
Adenosine
Assays
High Pressure Liquid Chromatography
Creatinine
Metabolic Diseases
Respiratory Insufficiency
Switches
Recovery
Molecules
Fluids

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Taniai, Hiroko ; Sumi, Satoshi ; Ito, Tetsuya ; Ueta, Akihito ; Ohkubo, Yumiko ; Togari, Hajime. / A simple quantitative assay for urinary adenosine using column-switching high-performance liquid chromatography. In: Tohoku Journal of Experimental Medicine. 2005 ; Vol. 208, No. 1. pp. 57-63.
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A simple quantitative assay for urinary adenosine using column-switching high-performance liquid chromatography. / Taniai, Hiroko; Sumi, Satoshi; Ito, Tetsuya; Ueta, Akihito; Ohkubo, Yumiko; Togari, Hajime.

In: Tohoku Journal of Experimental Medicine, Vol. 208, No. 1, 02.12.2005, p. 57-63.

Research output: Contribution to journalArticle

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