A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

Takayuki Murata, F. Goshima, T. Koshizuka, H. Takakuwa, Y. Nishiyama

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.

Original languageEnglish
Pages (from-to)1039-1043
Number of pages5
JournalJournal of Virology
Volume75
Issue number2
DOIs
Publication statusPublished - 16-01-2001
Externally publishedYes

Fingerprint

Human herpesvirus 1
Human Herpesvirus 1
amino acid substitution
Simplexvirus
Amino Acid Substitution
herpes simplex
Serial Passage
viruses
Cell Nucleus Active Transport
Viral DNA
Nuclear Proteins
Proteins
proteins
Viruses
Genes
nucleotide sequences
leptomycin B
genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.",
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A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B. / Murata, Takayuki; Goshima, F.; Koshizuka, T.; Takakuwa, H.; Nishiyama, Y.

In: Journal of Virology, Vol. 75, No. 2, 16.01.2001, p. 1039-1043.

Research output: Contribution to journalArticle

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T1 - A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

AU - Murata, Takayuki

AU - Goshima, F.

AU - Koshizuka, T.

AU - Takakuwa, H.

AU - Nishiyama, Y.

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Y1 - 2001/1/16

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