A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

T. Murata; F. Goshima; T. Koshizuka; H. Takakuwa; Y. Nishiyama

doi:10.1128/JVI.75.2.1039-1043.2001

A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

T. Murata
, F. Goshima
, T. Koshizuka
, H. Takakuwa
, Y. Nishiyama

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.

Original language	English
Pages (from-to)	1039-1043
Number of pages	5
Journal	Journal of Virology
Volume	75
Issue number	2
DOIs	https://doi.org/10.1128/JVI.75.2.1039-1043.2001
Publication status	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Insect Science
Virology

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10.1128/JVI.75.2.1039-1043.2001

Cite this

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abstract = "Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.",

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AU - Murata, T.

AU - Goshima, F.

AU - Koshizuka, T.

AU - Takakuwa, H.

AU - Nishiyama, Y.

PY - 2001

Y1 - 2001

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AB - Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.

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DO - 10.1128/JVI.75.2.1039-1043.2001

M3 - Article

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A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

Abstract

All Science Journal Classification (ASJC) codes

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