A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes

Hiroka Aonuma, Aya Yoshimura, Tomomi Kobayashi, Kiyoshi Okado, Athanase Badolo, Bryce Nelson, Hirotaka Kanuka, Shinya Fukumoto

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2 gene of Plasmodium berghei and the cytochrome oxidase subunit I gene of Dirofilaria immitis in mosquitoes. This technique could detect as few as 100 P. berghei-infected red blood cell-equivalents or one D. immitis microfilaria. Moreover, individual species of parasites in mosquitoes could be identified when a mixture of fluorescently labeled primer sets was used. These findings suggest that the multiplex LAMP assay is sensitive and specific enough to identify parasite-bearing mosquitoes in areas where several diseases occur simultaneously. This procedure could increase the efficiency and effectiveness of arthropod-borne disease elimination programs.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalExperimental Parasitology
Issue number2
Publication statusPublished - 06-2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Immunology
  • Infectious Diseases


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