TY - JOUR
T1 - A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes
AU - Aonuma, Hiroka
AU - Yoshimura, Aya
AU - Kobayashi, Tomomi
AU - Okado, Kiyoshi
AU - Badolo, Athanase
AU - Nelson, Bryce
AU - Kanuka, Hirotaka
AU - Fukumoto, Shinya
PY - 2010/6/1
Y1 - 2010/6/1
N2 - Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2 gene of Plasmodium berghei and the cytochrome oxidase subunit I gene of Dirofilaria immitis in mosquitoes. This technique could detect as few as 100 P. berghei-infected red blood cell-equivalents or one D. immitis microfilaria. Moreover, individual species of parasites in mosquitoes could be identified when a mixture of fluorescently labeled primer sets was used. These findings suggest that the multiplex LAMP assay is sensitive and specific enough to identify parasite-bearing mosquitoes in areas where several diseases occur simultaneously. This procedure could increase the efficiency and effectiveness of arthropod-borne disease elimination programs.
AB - Vector-borne diseases, such as malaria and lymphatic filariasis, are co-endemic in large parts of the world. To develop a multiplex amplification method for the simultaneous detection of multiple insect-borne infectious diseases, we used LAMP with fluorescently labeled primers to identify the SPECT2 gene of Plasmodium berghei and the cytochrome oxidase subunit I gene of Dirofilaria immitis in mosquitoes. This technique could detect as few as 100 P. berghei-infected red blood cell-equivalents or one D. immitis microfilaria. Moreover, individual species of parasites in mosquitoes could be identified when a mixture of fluorescently labeled primer sets was used. These findings suggest that the multiplex LAMP assay is sensitive and specific enough to identify parasite-bearing mosquitoes in areas where several diseases occur simultaneously. This procedure could increase the efficiency and effectiveness of arthropod-borne disease elimination programs.
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UR - http://www.scopus.com/inward/citedby.url?scp=77951893194&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2009.12.023
DO - 10.1016/j.exppara.2009.12.023
M3 - Article
C2 - 20064511
AN - SCOPUS:77951893194
VL - 125
SP - 179
EP - 183
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 2
ER -