A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA

Seiji Imamura; Nobuo Kido; Michio Kato; Hidetsugu Kawase; Akio Miyama; Takao Tsuji

doi:10.1016/S0378-1097(96)00483-1

A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA

Seiji Imamura, Nobuo Kido, Michio Kato, Hidetsugu Kawase, Akio Miyama, Takao Tsuji

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We detected Ent plasmids in 300 strains of human enterotoxigenic Escherichia coli, but one strain, E. coli 240-3, had neither a small nor a large plasmid and encoded the heat-labile enterotoxin (LTh(240-3)) gene on its chromosome. DNA sequences showed that LTh(240-3) differed by 12 and 14 base pairs from LT (LTh) and LT (LTp) from human H10407 and porcine EWD299 strains, respectively. In deduced precursor toxins, LTh(240-3), LTh and LTp differed from LTh, LTp and LTh(240-3) at nine, eight and eleven positions, respectively. These data suggest that although LTh(240-3) encoded in the chromosome is antigenically similar to LTh, it cannot be grouped with LTh due to differences in its DNA and amino acids sequences.

Original language	English
Pages (from-to)	241-245
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	146
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(96)00483-1
Publication status	Published - 15-01-1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Medicine

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10.1016/S0378-1097(96)00483-1

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title = "A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA",

abstract = "We detected Ent plasmids in 300 strains of human enterotoxigenic Escherichia coli, but one strain, E. coli 240-3, had neither a small nor a large plasmid and encoded the heat-labile enterotoxin (LTh(240-3)) gene on its chromosome. DNA sequences showed that LTh(240-3) differed by 12 and 14 base pairs from LT (LTh) and LT (LTp) from human H10407 and porcine EWD299 strains, respectively. In deduced precursor toxins, LTh(240-3), LTh and LTp differed from LTh, LTp and LTh(240-3) at nine, eight and eleven positions, respectively. These data suggest that although LTh(240-3) encoded in the chromosome is antigenically similar to LTh, it cannot be grouped with LTh due to differences in its DNA and amino acids sequences.",

author = "Seiji Imamura and Nobuo Kido and Michio Kato and Hidetsugu Kawase and Akio Miyama and Takao Tsuji",

note = "Funding Information: This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan.",

year = "1997",

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doi = "10.1016/S0378-1097(96)00483-1",

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T1 - A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA

AU - Imamura, Seiji

AU - Kido, Nobuo

AU - Kato, Michio

AU - Kawase, Hidetsugu

AU - Miyama, Akio

AU - Tsuji, Takao

N1 - Funding Information: This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Science and Culture of Japan.

PY - 1997/1/15

Y1 - 1997/1/15

N2 - We detected Ent plasmids in 300 strains of human enterotoxigenic Escherichia coli, but one strain, E. coli 240-3, had neither a small nor a large plasmid and encoded the heat-labile enterotoxin (LTh(240-3)) gene on its chromosome. DNA sequences showed that LTh(240-3) differed by 12 and 14 base pairs from LT (LTh) and LT (LTp) from human H10407 and porcine EWD299 strains, respectively. In deduced precursor toxins, LTh(240-3), LTh and LTp differed from LTh, LTp and LTh(240-3) at nine, eight and eleven positions, respectively. These data suggest that although LTh(240-3) encoded in the chromosome is antigenically similar to LTh, it cannot be grouped with LTh due to differences in its DNA and amino acids sequences.

AB - We detected Ent plasmids in 300 strains of human enterotoxigenic Escherichia coli, but one strain, E. coli 240-3, had neither a small nor a large plasmid and encoded the heat-labile enterotoxin (LTh(240-3)) gene on its chromosome. DNA sequences showed that LTh(240-3) differed by 12 and 14 base pairs from LT (LTh) and LT (LTp) from human H10407 and porcine EWD299 strains, respectively. In deduced precursor toxins, LTh(240-3), LTh and LTp differed from LTh, LTp and LTh(240-3) at nine, eight and eleven positions, respectively. These data suggest that although LTh(240-3) encoded in the chromosome is antigenically similar to LTh, it cannot be grouped with LTh due to differences in its DNA and amino acids sequences.

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DO - 10.1016/S0378-1097(96)00483-1

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

A unique DNA sequence of human enterotoxigenic Escherichia coli enterotoxin encoded by chromosomal DNA

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